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BIOMOD2012 Team Sendai
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<div style="float:left;width:5px;height:30px;">
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<h1>Team Sendai</h1>
<p>Tohoku University</p>
</div>
</div>
<a href="http://openwetware.org/wiki/%E5%9F%BA%E7%A4%8E%E3%82%BC%E3%83%9F%E3%83%81%E3%83%BC%E3%83%A0/basic_seminar_team"><div id="HOME">
HOME
</div></a>
<a href="http://openwetware.org/wiki/%E5%9F%BA%E7%A4%8E%E3%82%BC%E3%83%9F%E3%83%81%E3%83%BC%E3%83%A0/basic_seminar_team/project"><div id="bottun">
Project
</div></a>
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Experiment
</div></a>
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Diary
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Team
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Link
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<!—トップメニュー -->
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<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top">Top</a></li>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a></li>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Simulation">Simulation</a></li>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Results_%26_Discussion">Experiment</a></li>
<li><a href=" http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai/Movie">Diary</a></li>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Member ">Team</a></li>
</ul>


<!-- コンテンツ -->
<div id="Content">


</div>
<h2>Abstract</h2>


<p>
&nbsp;All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.　Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide,　the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
</p>
<img src="http://openwetware.org/images/4/46/Format_D-Heart.jpg" alt="D-Heart" width="790" height="602"/>


<p>
<strong>Selector</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target,　the target is passed to the inner-ones one by one.</br>
<strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br>
<strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.</br>
</p>


<h2>Movie</h2>
<p>Coming soon</p>


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<font id="head">Introduction</font>
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<ul id="Menu2">
<div id="line6"></div>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top">Top</a></li>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a></li>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Simulation">Simulation</a></li>
<li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Results_%26_Discussion">Experiment</a></li>
<li><a href=" http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai/Movie">Diary</a></li>
<li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Member ">Team</a></li>
</ul>


<div id="yohaku"></div>
<!-- フッター -->
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<div id="Footer">
<div id="kiji">
<address>Copyright (C) Team Sendai, All rights reserved.</address>
Recent DNA robots just wait for the target coming.
We are now developing a robot that can catch and store the target you like.
We named the robot D-NApper.
<br>
<br>
<div style="text-align:center;">
<div>
<img src="http://openwetware.org/images/thumb/9/95/Recent_robot.png/800px-Recent_robot.png" width="330px" height="224px">
<img src="http://openwetware.org/images/thumb/a/aa/Dnapperimage.png/800px-Dnapperimage.png" width="405px" height="224px">
</div>
</div>
</div>
<br>


To realize D-NApper, we propose "Selector" structure.<br>
<a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai">Legacy--Team Sendai B HP</a>
"Selector" catches a target distant from D-NApper, and puts it inside, with specificity.<br>
D-NApper can catch various molecules if we modify "Selector".
</div>


<!-- ここまでイントロダクション -->
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<div id="line1"></div>
<div id="line2"></div>
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<font id="head">Abstract</font>
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<div id="line4"></div>
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<div id="kiji">
We're making a molecular robot that can capture an object DNA and put it inside the robot.<br>
Our robot has the function we named "Selector".<br>
<br>
"Selector" is single-stranded DNA which has complementary sequences to the "Target DNA" here and there, and consecutive adenine sequences in other portion. We have three "Selectors", and the inner "Selector" attaches to the target more strongly. When "Selector 1" catches the "Target DNA", the left sequences (which are not complementary and do not attach to the object) make loops. Then the structure shrinks and becomes shorter.<br>
<br>
Thanks to "Selector 1", the robot can select and draw the target DNA close to the robot. Next, "Selector 2" receives the target DNA from "Selector 1". And "Selector 3" receives the target from "Selector 2". Thanks to "Selector 2" and "Selector 3", the robot can deliver only the object inside certainly.<br>
<br>
Then, we will explain the body structure containing these functions.<br>
We design the robot like a tube of hexagonal base. In case it is too difficult to form and observe, we also design a triangle prism body. As for the latter shape, we made the same kind of structure last year. We've acquired some know-how of it.<br>
<br>
We think the robot should have a lid so that nothing would come in from the back. So we arrange "Selectors" symmetrically. <br>
</div>
<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top">Legacy--Team Sendai A HP</a>
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Revision as of 02:17, 13 October 2012

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<!-- コンテナ --> <div id="Container">

<!-- ヘッダー --> <div id="Header"> <h1>Team Sendai</h1> <p>Tohoku University</p> </div>

<!—トップメニュー --> <ul id="Topmenu"> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top">Top</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Simulation">Simulation</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Results_%26_Discussion">Experiment</a></li> <li><a href=" http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai/Movie">Diary</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Member ">Team</a></li> </ul>

<!-- コンテンツ --> <div id="Content">

<h2>Abstract</h2>

<p> &nbsp;All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.　Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide,　the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane. 

</p>

<img src="http://openwetware.org/images/4/46/Format_D-Heart.jpg" alt="D-Heart" width="790" height="602"/>

<p> <strong>Selector</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target,　the target is passed to the inner-ones one by one.</br> <strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br> <strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.</br> </p>

<h2>Movie</h2> <p>Coming soon</p>

</div>

<!—フッターメニュー --> <ul id="Menu2"> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top">Top</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Project">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Simulation">Simulation</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Results_%26_Discussion">Experiment</a></li> <li><a href=" http://openwetware.org/wiki/BIOMOD/2012/Team_Sendai/Movie">Diary</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Member ">Team</a></li> </ul>

<!-- フッター --> <div id="Footer"> <address>Copyright (C) Team Sendai, All rights reserved.</address> </div> 

<a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai">Legacy--Team Sendai B HP</a>

</div>

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