Biomod/2012/Tianjin/Result/Origami: Difference between revisions
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<ul | <ul><li> <a href="http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/LogicGate" title="LogicGate">The Logic Gate</a><div class="tianjin-description">Looking at this design, there are two critical problems regarding our design</div> | ||
</li><li> <a href="http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/YDNA" title="YDNA">Y-DNA</a><div class="tianjin-description">The Y-DNA was synthesized from three ssDNAs, each of which has partial complementary sequences to the other two ssDNAs.</div> | </li><li> <a href="http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/YDNA" title="YDNA">Y-DNA</a><div class="tianjin-description">The Y-DNA was synthesized from three ssDNAs, each of which has partial complementary sequences to the other two ssDNAs.</div> | ||
</li><li> <strong class="selflink">The Origami Amplifier</strong><div class="tianjin-description">In this design, we focus on two essential problems.</div> | </li><li> <strong class="selflink">The Origami Amplifier</strong><div class="tianjin-description">In this design, we focus on two essential problems.</div> | ||
Revision as of 18:08, 27 October 2012
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<div id="header"> <div class="title" style="height: 180px;"> <a href="http://openwetware.org/wiki/Biomod/2012/Tianjin"><img src ="http://openwetware.org/images/7/79/TJU2012-Header.png"></a> <div style="float:right;"><img src="http://openwetware.org/images/c/cd/TJU2012-logo.png"></div> <div class="topsearch" style="position:absolute;top:175px;right:90px;"> <form method="GET" action="http://www.google.com/search"> <input type="hidden" name="hl" value="en"> <input type="hidden" name="sitesearch" value="openwetware.org"> <input type="hidden" name="domains" value="openwetware.org"> <input type="hidden" name="as_epq" value="Tianjin"> <input type="text" name="q" class="frm"><input type="submit" class="btn" value="Search"> </form> </div> </div> </div>
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<li class='active '><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin'><span>Home</span></a></li>
<li class='active '><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin/Team'><span>Team</span></a>
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<li class='has-sub'><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin/Project/Background'><span>Designs</span></a>
<ul>
<li><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin/Project/Background'><span>Background</span></a></li>
<li><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin/Project/LogicGate'><span>The Logic Gate</span></a></li>
<li><a href="http://openwetware.org/wiki/Biomod/2012/Tianjin/Project/YDNA"><span>Y-DNA</span></a></li>
<li><a href="http://openwetware.org/wiki/Biomod/2012/Tianjin/Project/Origami"><span>The Origami Amplifier</span></a></li>
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<li class='has-sub'><a href='#'><span>Experiments and Results</span></a>
<ul>
<li><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/LogicGate'><span>The Logic Gate</span></a></li>
<li><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/YDNA'><span>Y-DNA</span></a></li>
<li><a href='http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/Origami'><span>The Origami Amplifier</span></a></li>
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<div id="tianjin-right-content"> <ul><li> <a href="http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/LogicGate" title="LogicGate">The Logic Gate</a><div class="tianjin-description">Looking at this design, there are two critical problems regarding our design</div> </li><li> <a href="http://openwetware.org/wiki/Biomod/2012/Tianjin/Result/YDNA" title="YDNA">Y-DNA</a><div class="tianjin-description">The Y-DNA was synthesized from three ssDNAs, each of which has partial complementary sequences to the other two ssDNAs.</div> </li><li> <strong class="selflink">The Origami Amplifier</strong><div class="tianjin-description">In this design, we focus on two essential problems.</div> </li></ul> </div>
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<div id="outborder" style="background: #FAFAFA url(http://biomod.net/sites/all/themes/biomod/images/stripe.png) repeat; height:360px; width:640px; padding:10px;"> <div id="inborder" style="background: #fff;height:340px;width:620px;padding:10px;"> <a href="http://openwetware.org/index.php?title=Biomod/2012/Tianjin/Result/Origami&action=edit">this page edit</a> <div id="frametext"><br><br><span style="font-size:300%;">In</span> this design, we focus on two essential problems: 1. Is our design able to load cargo? 2. Is 8-17 DNAzyme still capable of multiple turnover in our new design? We subsequently answered the questions in the experiment.</div> <div id="frameimg"><img src="http://openwetware.org/images/9/92/TJU2012-frame-ROr.png"></div> </div> </div>
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Experiment & Results
- The staple strands and M13MP18 were annealed in PCR, and formed the origami container.
- We verified the multiple turnover of 8-17 in the logic gate. In the solution, we added 5μM reporter and 1μM 8-17. From Figure X, we could notice that more than 1 uM substrate was cleaved, which represents the multiple turnover character.
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