Biomod/2012/Titech/Nano-Jugglers/Methods: Difference between revisions
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::''' | ::'''DNA conjugation to polystyrene surface'''<br> | ||
Carboxyl (COOH) microparticles can be used for covalent coupling of proteins by activating the carboxyl group with water-soluble carbodiimide. The carbodiimide reacts with the carboxyl group to create an active ester that is reactive toward primary amines on the DNA.<br> | Carboxyl (COOH) microparticles can be used for covalent coupling of proteins by activating the carboxyl group with water-soluble carbodiimide. The carbodiimide reacts with the carboxyl group to create an active ester that is reactive toward primary amines on the DNA.<br> | ||
1.Carboxyl group of polystyrene beads and EDAC react with each other<br> | 1.Carboxyl group of polystyrene beads and EDAC react with each other<br> | ||
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::''' | ::'''DNA conjugation to metal surface'''<br> | ||
When we mix thiol-modified DNA and Pt or Au, they will be self assembly. Thiol-modified DNA and Au or Pt are organized naturally. Using this characteristic, bond two kinds of DNA with Pt and bond another kind of DNA with Au.<br><br> | When we mix thiol-modified DNA and Pt or Au, they will be self assembly. Thiol-modified DNA and Au or Pt are organized naturally. Using this characteristic, bond two kinds of DNA with Pt and bond another kind of DNA with Au.<br><br> | ||
Bond photoresponsive DNA with Au and bond normal DNA with Polystyrene. When we add Pt which has complementary DNA, we can connect to the body of our rocket with Pt regiospecifically. | Bond photoresponsive DNA with Au and bond normal DNA with Polystyrene. When we add Pt which has complementary DNA, we can connect to the body of our rocket with Pt regiospecifically. |
Revision as of 12:37, 22 October 2012
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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>
0.Construction of Biomolecular Rocket
In order to have a positional specificity, We make metal-deposited beads. Hemispherical part of polystyrene bead is covered with Cr,1/4 part of spere is covered with Au and the other part is polystyrene. |
<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/3/3e/BIOMODTNJPVD.png" border=0 width=400 height=130></a></td></body></html> |
Carboxyl (COOH) microparticles can be used for covalent coupling of proteins by activating the carboxyl group with water-soluble carbodiimide. The carbodiimide reacts with the carboxyl group to create an active ester that is reactive toward primary amines on the DNA. |
<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/2/2c/BIOMODTNJEDAC.png" border=0 width=300 height=280></a></td></body></html> |
When we mix thiol-modified DNA and Pt or Au, they will be self assembly. Thiol-modified DNA and Au or Pt are organized naturally. Using this characteristic, bond two kinds of DNA with Pt and bond another kind of DNA with Au. |
<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/0/01/BIOMODTNJSAM3.png" border=0 width=300 height=240></a></td></body></html> |
Finally, we conjugated the catalyst to our rocket body by DNA hybridization. Complementary strand DNA had adhered to the corresponding parts of the rocket body, so it enabled to conjugate catalyst engines in that we designed these DNA were thermodynamically stable when they hybridized.
we build our rocket in this way. |
<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/1/19/Conjugation_catalyst.jpg" border=0 width=300 height=240></a></td></body></html> 0-1.Selective coating of the body
0-2.DNA design
0-3.DNA conjugation0-3-1.Selective conjugation of DNA to polystyrene surface area
0-3-2.Selective conjugation of DNA to metal surface area
0-4.Catalyst conjugation by DNA hybridization
1.Power supply for the rail-free movement
1-1.DNA hybridization in solution of H2</subl>O2
1-2.Verification of platinum hemisphere behavior in solution of H2O2
1-3.Verification of catalase hemisphere behavior in solution of H2O2
2.Increasing driving force for the high-speed movement
2-1.Analyses of the speed of platinum in solution of H2O2 by High-speed camera
3.Introduction of a photo-switchable DNA system for the directional control
3-1.Design of photoresponsive DNA
We incorporated 4 azobenzene units into photoresponsive DNA strand so that we can quickly dissociated DNA duplex.{Photo-switchable DNA 5'-AATXACXCCXAGXCC-3' (X=Azobenzene)}
3-2.Dissociation of photoresponsive DNA by UV-light irradiation
DNA design
Ascertain the photo-switching system
|