Biomod/2012/Titech/Nano-Jugglers/Methods/Vapor Deposition: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 9: Line 9:
::Deposition is a method for forming a metal thin film on the surface of the substrate.
::Deposition is a method for forming a metal thin film on the surface of the substrate.
::In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate specific DNA onto the determined location on the surface.
::In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate specific DNA onto the determined location on the surface.
==Method==
==Physical Vapor Deposition Method==
{|
:[[Image: BIOMODPVDM1.png]]
|
===1st Deposition===
===1st Deposition===
::First, we deposit gold on the polystyrene-bead and make bead, which are deposited by gold half.
::First, we deposit gold on the polystyrene-bead and make bead, which are deposited by gold half.
Line 22: Line 25:
::Therefore, platinum particles can't conjugate onto chromium hemisphere.
::Therefore, platinum particles can't conjugate onto chromium hemisphere.
::As a result, JET starts to move toward chromium hemisphere heading.
::As a result, JET starts to move toward chromium hemisphere heading.
|}

Revision as of 07:03, 2 September 2012


<html> <head> <style type="text/css"> /* ====================

  主に全体に関わるCSS

   ==================== */ body.mediawiki {

                       font-size: 14px;

background-image:url(http://openwetware.org/images/2/2d/TNJback.png); background-repeat: repeat; font-family: Calibri, Verdana, helvetica, sans-serif; }

               h1 {
                       padding: 0px 20px 5px 20px;
                       font-size: 34px;
                       line-height: 40px;
                       font-weight: bold;
               }
               h2 {
                       padding: 20px 20px 5px 20px;
                       font-size: 25px;
                       color: #000000;
                       text-decoration: none;
                       font-weight: bold; 
              }
               h2 a {
                       color: #eb8300;
               }
               h3 {
                       padding: 20px 20px 5px 20px;
                       font-size: 20px;
                       color: #000;
                       font-decoration: none;
                       font-weight: bold;
               }
               h1.firstHeading {

display: none;

               }

p { text-align: justify; } a:link { color: #00a5ea; text-decoration: none } a:visited { color:#00a5ea; text-decoration: none } a:hover {

                       color: #eb8300;

text-decoration: none } a:active { color:#f29400; text-decoration: none } #content{ margin: 0px 0 0px 0; align: center; padding: 12px 12px 12px 12px; width: 946px; background-color: #ffff; border: 0; } #bodyContent{ width: 970px; margin: 150px auto 0 auto; align: center; background-color: #ffffff; } #globalWrapper{ margin: 20px 0 0 0; width:994px; background-color: #ffffff; margin-left: auto; margin-right: auto } #biomodlink{ position:absolute;top:0px; width:970px;height:20px; background-color: #ffffff; margin:3px auto 3px auto }


/* ====================

  メニューの画像を変更できる部分
  ==================== */

#header {

                       margin: 0 auto 0 auto;
                       position:absolute;
                       top:28px; width:968px;height:189px;

background-color: #FFFFFF; background-repeat: no-repeat;

                       background-image: url(http://openwetware.org/images/7/74/Title-LOGO6.jpg); 

} /* ====================

  以下、特殊なclassに適用される
  ==================== */

#navigation { position:absolute;

                       top:120px; width:970px; height:92px;
                       background-repeat: no-repeat;

color: #0000FF; }

/* ====================

  ここからプルダウン周辺のデザイン
  ==================== */
  1. menu * {

margin: 0; padding: 0; }

  1. menu {
       position:absolute;

font-family: calibri, verdana, sans-serif;

       font-color: #000000;

background-color: transparent; float:left; display: block; }

  1. menu ul {
       position:relative;

float: left; text-align:center; list-style: none; }

  1. menu li {

background-color:yellow;

       position:relative;

float:left;

       width: 136px;

font-size: 23px;

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu li.ach {

background-color:yellow;

       position:relative;

float:left;

       width: 162px;

font-size: 23px;

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu li.sup {

background-color:yellow;

       position:relative;

float:left;

       width: 109px;

font-size: 17px;

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu li.none {

background-color:yellow;

       position:relative;

float:left;

       width: 152px;

font-size: 17px;

       font-weight: bold;

border:ridge 0px #FFFFFF; }

  1. menu a {

color: #000000; display:block; text-decoration: none; }

  1. menu a:hover {

background-color:gold; }

  1. column-one { display:none; width:0px;}

.container{background-color: #ffffff; margin-top:0px} .OWWNBcpCurrentDateFilled {display: none;}

  1. footer{position: center; width: 994px}

@media screen {

   body { background: #F5F5F5 0 0 no-repeat;  /* changed default background */ }

} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>


Physical Vapor Deposition Method

>>Result page is here

Overview

Deposition is a method for forming a metal thin film on the surface of the substrate.
In this project, Gold and Chromium were deposited on the polystyrene surface in order to conjugate specific DNA onto the determined location on the surface.

Physical Vapor Deposition Method

1st Deposition

First, we deposit gold on the polystyrene-bead and make bead, which are deposited by gold half.

2nd Deposition

Next, we change the direction of the body, and deposit chromium on the body.
Then we pick up polystyrene-bead, which are deposited by quarter gold and half chromium.

After PVD

Through twice of physical vapor deposition,
  • we conjugate thiol modified DNA onto gold part.
  • we conjugate amino modified DNA onto polystyrene part.
  • There are no DNA staple strands on chromium hemisphere.
Therefore, platinum particles can't conjugate onto chromium hemisphere.
As a result, JET starts to move toward chromium hemisphere heading.