Biomod/2012/Tohoku/Team Sendai

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<h1 style="margin-top:-50px;"><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1>
 
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<p>Tohoku University</p>
 
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<!—二段組み本文 -->
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<text-align="center"><h1>We want to create a new channel made of DNA!!(躍動感のあるフォントが欲しい)</h1>
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<h1 align="center";><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1>
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<h1>Abstract</h1>
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We want to create a channel that does not exist in nature. DNA is a designable molecule and has interaction with various biomolecules. Thus, a channel made of DNA enables to transport more various biomolecules against energy gradient.
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All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.</br>
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Such a new channel will be used…</br>
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A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
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1. In medical care, because it can transport new molecules that are unable to be transported into cells without DNA.</br>
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Here, we decided to create a novel device with such a function. </br>
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2. For sampling what we want from cells.</br>
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We have designed <strong>a cylindrical injector/extractor device made of DNA origami</strong> (we named this device: <strong>CELL-GATE</strong>). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
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3. For BIOROOMBA by compositing with artificial liposome.( Off course use for DDS)
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We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
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(図が欲しい)</br>
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<h2><!--Abstract-->Project Image</h2>
<h2><!--Abstract-->Project Image</h2>
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<img src="http://openwetware.org/images/4/46/Format_D-Heart.jpg" alt="D-Heart" width="400px"><br>
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<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>
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<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called  Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.</br>
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<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
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<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
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<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px" usemap="#D-Heart">
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<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter">
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<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate">
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<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE" alt="Membarane">
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Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!
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<h2>Movie</h2>
<h2>Movie</h2>
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<p>Coming soon</p>
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<td style="color:white;">Coming soon...</td>
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<h1 style="width:900px;clear:left;margin:15px;">
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Abstract</h1>
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&nbsp;All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.
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A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.<br>
+
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Here, we decided to create a novel device with such a function. <br>
+
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We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.<br>
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We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.<br>
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<br>
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<strong>Selector</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one.</br>
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<strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br>
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<strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.</br>
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<a href=" http://openwetware.org/wiki/Biomod/2012/Team_Sendai_Old">Test page</a>
 
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Current revision


Team Sendai Top

Abstract

All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.
Here, we decided to create a novel device with such a function.
We have designed a cylindrical injector/extractor device made of DNA origami (we named this device: CELL-GATE). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.

Project Image

Our PROJECT CELL-GATE is divided into three sub-projects:
PORTER: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.
GATE: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.
MEMBRANE: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.

D-Heart Porter Gate Membarane Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!

Movie



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