All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.
Here, we decided to create a novel device with such a function.
We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
Selector: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one. Gate: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor. Membrane: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
僕たちはＤＮＡでチャネルを作りたい。天然には存在しない完ぺきな人工チャネルを作りたい！ もしこれが出来ると・・・ ・実際に存在するチャネルでは運べなかったけれど細胞内に運びたいものを運ぶことが出来る！→医療にも応用できるかも ・細胞内から欲しい物質を捕ってくることが出来る！ ・自分たちで作ったリポソームまで含めることで体内でいらない物質を取り込みきれいにするお掃除ロボットにもなる！（もちろん薬をまく噴霧ロボットにも） だから僕たちはＤＮＡロボットを作るんだ！！
We want to create a channel working in a new way that does not exist in nature. The channel uses DNA characteristic. DNA is a designable molecule and has interaction with various biomolecules. So if we create a new channel made of DNA, we transport more various biomolecules and create intentionally energy gradient. This new channel will be used… 1. In medical care, because it can transport new molecules that are unable to be transported into cells without DNA. 2. For sampling what we want from cells. 3. For cleaning or DDS by compositing with artificial liposome. （図が必要）Test page