All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes. A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell. Here, we decided to create a novel device with such a function. We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones. We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.
PORTER: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is hereinafter called Porter. Once the outer-most Porter binds to a target DNA, the target passes to the inner-ones Porters one by one. GATE: Cylindrical nanostructure connecting the inside and outside of MEMBRANE like a gate. A cascade of the Porters is planted inside this cylinder. We can use this as an injector or extractor. MEMBRANE: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane for a cell.