Biomod/2012/UCSD/tRiton Nano Architects: Difference between revisions

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=Welcome to tRiton Nano Architects's BIOMOD Wiki!=
=Abstract: Fluorescent DNA Aptamer for lysozyme detection =
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=Abstract: Fluorescent DNA Aptamer for lysozyme detection =


Combining previous work on structure-switching signaling aptamers and fluorescent signaling we design a DNA based aptamer modified with fluorophores and quenchers to detect and signal the presence of lysozyme. This device is then compared against the same attached with a nanoparticle. We verify the device’s behavior using polyacrylamide gel electrophoresis, atomic force microscopy, and fluorescent spectroscopy. Our device demonstrates that DNA nano-structures can be designed to meet detection of specific and trace quantities of proteins and this can have important applications such as low cost sensors for detecting deadly viruses via viral capsid proteins.
Combining previous work on structure-switching signaling aptamers and fluorescent signaling we design a DNA based aptamer modified with fluorophores and quenchers to detect and signal the presence of lysozyme. This device is then compared against the same attached with a nanoparticle. We verify the device’s behavior using polyacrylamide gel electrophoresis, atomic force microscopy, and fluorescent spectroscopy. Our device demonstrates that DNA nano-structures can be designed to meet detection of specific and trace quantities of proteins and this can have important applications such as low cost sensors for detecting deadly viruses via viral capsid proteins.
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[[Biomod/2012 | Back to the Biomod 2012 webpage]]  
[[Biomod/2012 | Back to the Biomod 2012 webpage]]
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= References template =
*Brown, R (2006). "Size of the Moon", ''Scientific American'', 51(78).
*Miller, E (2005). ''The Sun'', Academic Press.

Latest revision as of 22:37, 27 October 2012

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        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects#Abstract:_Fluorescent_DNA_Aptamer_for_lysozyme_detection'><span>Abstract</span></a></li>
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Abstract: Fluorescent DNA Aptamer for lysozyme detection

Combining previous work on structure-switching signaling aptamers and fluorescent signaling we design a DNA based aptamer modified with fluorophores and quenchers to detect and signal the presence of lysozyme. This device is then compared against the same attached with a nanoparticle. We verify the device’s behavior using polyacrylamide gel electrophoresis, atomic force microscopy, and fluorescent spectroscopy. Our device demonstrates that DNA nano-structures can be designed to meet detection of specific and trace quantities of proteins and this can have important applications such as low cost sensors for detecting deadly viruses via viral capsid proteins.

Video


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