Biomod/2012/UCSD/tRiton Nano Architects/Project introduction: Difference between revisions
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= | =Concepts= | ||
==What is an Aptamer?== | |||
An aptamer is a stable DNA or RNA molecule that has been selected from random pools based on it’s ability to bind with high affinity to target molecules such as small organics, proteins, and even entire cells. Unlike antibodies, aptamers are selected in vitro, are capable of higher specificity and affinity, can tolerate pH and temperatures that their protein counterparts cannot. These features allow them to be highly specific detectors and to be stored at ambient temperatures with much higher shelf life. | |||
==References== | |||
1. Stoltenburg R, Reinemann C, Strehlitz B: SELEX–a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomolecular engineering 2007, 24:381-403. | |||
2. Jayasena SD: Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics. Clinical Chemistry 1999, 45:1628-1650. | |||
Revision as of 23:04, 27 October 2012
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<li class='active has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><span>Home</span></a> <ul> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects#Abstract:_Fluorescent_DNA_Aptamer_for_lysozyme_detection'><span>Abstract</span></a></li> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects#Video'><span>Video</span></a></li> </ul> </li> <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Project_introduction'><span>Project Introduction</span></a> <ul> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Project_introduction#Concepts'><span>Concepts</span></a></li> </ul> </li> <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Experiment'><span>Experiment</span></a> <ul> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Experiment#Methods'><span>Methods</span></a></li> </ul> </li> <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Resources'><span>Supplementary Resources</span></a> <ul> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Brainstorm#DNA_architecture_log'><span>DNA Architecture Log</span></a></li> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Brainstorm#Project_Ideas'><span>Project Ideas</span></a></li> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Literature'><span>Literature</span></a></li> </ul> </li> <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Team'><span>Team</span></a></li>
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Concepts
What is an Aptamer?
An aptamer is a stable DNA or RNA molecule that has been selected from random pools based on it’s ability to bind with high affinity to target molecules such as small organics, proteins, and even entire cells. Unlike antibodies, aptamers are selected in vitro, are capable of higher specificity and affinity, can tolerate pH and temperatures that their protein counterparts cannot. These features allow them to be highly specific detectors and to be stored at ambient temperatures with much higher shelf life.
References
1. Stoltenburg R, Reinemann C, Strehlitz B: SELEX–a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomolecular engineering 2007, 24:381-403.
2. Jayasena SD: Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics. Clinical Chemistry 1999, 45:1628-1650.