Biomod/2012/UCSD/tRiton Nano Architects/Project introduction

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==References==
==References==
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1. Stoltenburg R, Reinemann C, Strehlitz B: SELEX–a (r)evolutionary method to generate high-affinity nucleic acid ligands. Biomolecular engineering 2007, 24:381-403.
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1. Stoltenburg, Regina, Christine Reinemann, and Beate Strehlitz. "SELEX? A (r) evolutionary method to generate high-affinity nucleic acid ligands." Biomolecular engineering 24.4 (2007): 381-403.
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2. Jayasena SD: Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics. Clinical Chemistry 1999, 45:1628-1650.
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2. Jayasena, Sumedha D. "Aptamers: an emerging class of molecules that rival antibodies in diagnostics." Clinical Chemistry 45.9 (1999): 1628-1650.

Revision as of 02:09, 28 October 2012

Contents

Concepts

What is an Aptamer?

An aptamer is a stable DNA or RNA molecule that has been selected from random pools based on it’s ability to bind with high affinity to target molecules such as small organics, proteins, and even entire cells. Unlike antibodies, aptamers are selected in vitro, are capable of higher specificity and affinity, can tolerate pH and temperatures that their protein counterparts cannot. These features allow them to be highly specific detectors and to be stored at ambient temperatures with much higher shelf life.

What is a fluorophore?

A fluorophore is a chemical group that fluoresces when exposed to a specific wavelength of light. Fluorophores can be attached to oligonucleotides to make it a fluorescent transducer. Popular green, red and blue fluorophores used in direct labeling are fluorescein, rhodamine, and amino methyl coumarin respectively.



References

1. Stoltenburg, Regina, Christine Reinemann, and Beate Strehlitz. "SELEX? A (r) evolutionary method to generate high-affinity nucleic acid ligands." Biomolecular engineering 24.4 (2007): 381-403.

2. Jayasena, Sumedha D. "Aptamers: an emerging class of molecules that rival antibodies in diagnostics." Clinical Chemistry 45.9 (1999): 1628-1650.


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