Biomod/2012/UT/Nanowranglers - Revision history

Ben Braun at 03:59, 28 October 2012

Ben Braun — Sun, 28 Oct 2012 03:59:43 GMT

←Older revision		Revision as of 03:59, 28 October 2012
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	=Acknowledgements=		=Acknowledgements=

-	We are sponsored by Integrated DNA Technologies (Coralville, IA, USA). We received travel support from the College of Natural Sciences, the University of Texas at Austin.	+	We are sponsored by Integrated DNA Technologies (Coralville, IA, USA). We received travel support from the College of Natural Sciences, the University of Texas at Austin and from the Ellington Lab.

	Background photo taken by Linhao Zhang in Fort Davis, Texas. Used with permission.		Background photo taken by Linhao Zhang in Fort Davis, Texas. Used with permission.

Jeremy R. McLain: /* Concept */

Jeremy R. McLain — Sun, 28 Oct 2012 03:52:30 GMT

Concept

←Older revision		Revision as of 03:52, 28 October 2012
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	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because F1:F2, see below for details, is free-floating in solution and has no preference to location as long as they are not interacting with the track or walker, we expect there to be leakage of the walkers to some extent. This is due to the F1:F2 complex reacting with the track to displace a walker leg while the other is still in transition. Although is creates a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss, because we can still learn useful information about this system that has not been obtained previously to our knowledge. Such as the fact that there ~~is not a good tool~~ available to predict some of the unique kinetics of our design. For more information concerning this please see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].	+	However, our design does sacrifice some functionality by using this system of fuels. Because F1:F2, see below for details, is free-floating in solution and has no preference to location as long as they are not interacting with the track or walker, we expect there to be leakage of the walkers to some extent. This is due to the F1:F2 complex reacting with the track to displace a walker leg while the other is still in transition. Although it creates a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss, because we can still learn useful information about this system that has not been obtained previously to our knowledge. Such as the fact that there are limited tools available to predict some of the unique kinetics of our design. For more information concerning this please see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].

	=Design and walking mechanism=		=Design and walking mechanism=

Jeremy R. McLain: /* Concept */

Jeremy R. McLain — Sun, 28 Oct 2012 03:50:47 GMT

Concept

←Older revision		Revision as of 03:50, 28 October 2012
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	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because F1:F2, see below for details, is free-floating in solution and has no preference to location as long as they are not interacting with the track or walker, we expect there to be leakage of the walkers to some extent. This is due to the F1:F2 complex reacting with the track to displace a walker leg while the other is still in transition. Although is creates a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss, because we can still learn useful information about this system that has not been obtained previously to our knowledge. Such as the fact that there is ~~currently no~~ tool available to predict some of the kinetics of our design. For more information concerning this please see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].	+	However, our design does sacrifice some functionality by using this system of fuels. Because F1:F2, see below for details, is free-floating in solution and has no preference to location as long as they are not interacting with the track or walker, we expect there to be leakage of the walkers to some extent. This is due to the F1:F2 complex reacting with the track to displace a walker leg while the other is still in transition. Although is creates a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss, because we can still learn useful information about this system that has not been obtained previously to our knowledge. Such as the fact that there is not a good tool available to predict some of the unique kinetics of our design. For more information concerning this please see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].

	=Design and walking mechanism=		=Design and walking mechanism=

Jeremy R. McLain: /* Concept */

Jeremy R. McLain — Sun, 28 Oct 2012 03:46:43 GMT

Concept

←Older revision		Revision as of 03:46, 28 October 2012
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	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because F1:F2, see below for details, is free-floating in solution and has no preference to location as long as they are not interacting with the track or walker, we expect there to be leakage of the walkers to some extent. This is due to ~~the method by which~~ the F1:F2 complex~~, see below for details, can displace~~ the ~~lagging~~ leg while the ~~leading leg~~ is still in transition ~~to its new foothold~~. Although ~~there~~ is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss~~. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We~~ can still learn useful information about this system that has not been obtained previously to our knowledge. ~~There~~ is no tool available to predict some of the kinetics of our design. For more information see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].	+	However, our design does sacrifice some functionality by using this system of fuels. Because F1:F2, see below for details, is free-floating in solution and has no preference to location as long as they are not interacting with the track or walker, we expect there to be leakage of the walkers to some extent. This is due to the F1:F2 complex reacting with the track to displace a walker leg while the other is still in transition. Although is creates a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss, because we can still learn useful information about this system that has not been obtained previously to our knowledge. Such as the fact that there is currently no tool available to predict some of the kinetics of our design. For more information concerning this please see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].

	=Design and walking mechanism=		=Design and walking mechanism=

Jeremy R. McLain: /* Concept */

Jeremy R. McLain — Sun, 28 Oct 2012 03:41:26 GMT

Concept

←Older revision		Revision as of 03:41, 28 October 2012
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	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because ~~all molecules are~~ free-floating in solution and ~~have~~ no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].	+	However, our design does sacrifice some functionality by using this system of fuels. Because F1:F2, see below for details, is free-floating in solution and has no preference to location as long as they are not interacting with the track or walker, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].

	=Design and walking mechanism=		=Design and walking mechanism=

Andy Tran: /* Concept */

Andy Tran — Sun, 28 Oct 2012 03:38:29 GMT

Concept

←Older revision		Revision as of 03:38, 28 October 2012
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	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [~~http://openwetware.org/wiki/~~Biomod/2012/UT/Nanowranglers/Results#~~Kinetic_simulation_of_walker~~ Results].	+	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [[Biomod/2012/UT/Nanowranglers/Results#Kinetic simulation of walker \| Results]].

	=Design and walking mechanism=		=Design and walking mechanism=

Andy Tran: /* Concept */

Andy Tran — Sun, 28 Oct 2012 03:33:20 GMT

Concept

←Older revision		Revision as of 03:33, 28 October 2012
Line 19:		Line 19:
	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [[Results]].	+	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [http://openwetware.org/wiki/Biomod/2012/UT/Nanowranglers/Results#Kinetic_simulation_of_walker Results].

	=Design and walking mechanism=		=Design and walking mechanism=

Andy Tran: /* Concept */

Andy Tran — Sun, 28 Oct 2012 03:31:34 GMT

Concept

←Older revision		Revision as of 03:31, 28 October 2012
Line 19:		Line 19:
	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [[Results]]	+	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [[Results]].

	=Design and walking mechanism=		=Design and walking mechanism=

Andy Tran: /* Concept */

Andy Tran — Sun, 28 Oct 2012 03:31:24 GMT

Concept

←Older revision		Revision as of 03:31, 28 October 2012
Line 19:		Line 19:
	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. ~~An example is this is~~ [[~~here~~]]	+	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. For more information see [[Results]]

	=Design and walking mechanism=		=Design and walking mechanism=

Jeremy R. McLain: /* Concept */

Jeremy R. McLain — Sun, 28 Oct 2012 03:25:37 GMT

Concept

←Older revision		Revision as of 03:25, 28 October 2012
Line 19:		Line 19:
	In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.		In order for this walker to be comparable to (or even better than) kinesin, it must be autonomous and run on a reusable track. In other words, it should not require an external driver and should not damage the track when taking steps. Other walkers have been created in the past, but none has yet been able to accomplish both of these tasks. There are some that are at least capable of either walking autonomously [2] or of reusing their track [63]. Our walker is able to accomplish both tasks by means of two alterations. The use of two discrete fuel strands which can only interact with the walker system in a specific order allow us to add an excess of fuel while still keeping the walker on the track. We also make use of a nicking enzyme which restores the track to its original form without harming it, allowing it to be reused.

-	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. ~~We expect the probability~~ of this ~~occurring to be very low~~ due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. An example is this is [[here]]	+	However, our design does sacrifice some functionality by using this system of fuels. Because all molecules are free-floating in solution and have no preference to location, we expect there to be leakage of the walkers to some extent. This is due to the method by which the F1:F2 complex, see below for details, can displace the lagging leg while the leading leg is still in transition to its new foothold. Although there is a chance that some part of the walker could dissociate from the track, we believe this is an acceptable loss. What causes this is due to the functional breakdown of F1:F2 by the nicking enzyme when the fuels successfully bind to the track. We can still learn useful information about this system that has not been obtained previously to our knowledge. There is no tool available to predict some of the kinetics of our design. An example is this is [[here]]

	=Design and walking mechanism=		=Design and walking mechanism=