Biomod/2012/UTokyo/UT-Hongo

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<p style="font-size: 100%; font-weight: bold; margin-left: auto; margin-right: auto; text-align: center">fig1. Schematic animation of DNA Shell</p>
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<p style="font-size: 100%; font-weight: bold; margin-left: auto; margin-right: auto; text-align: center">Fig1. Schematic animation of DNA Shell</p>
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       <p id="desc-first">Methodologies to apply DNA for building precisely controlled nanostructures have greatly developed over the recent years. Our focus for BIOMOD is to utilize DNA to make a shell like structure which can capture molecules inside the shell-like body, as if a shellfish is eating its prey. Entrapment of the substrate was detected by the numerous florescent molecules that are attached to the body. This research may become a foundation for using DNA to mimic catalytic activity of enzymes.</p>
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       <p id="desc-first">Our focus for BIOMOD is to utilize DNA to make a shell like structure which can capture molecules inside the body, as if a shellfish is capturing its prey. The device, named DNA Shell, can exhibit functionalities such as highly sensitive detection, substrate protection, and attachment to solid surfaces. All these new functionalities are the result of this new capturing mechanism that we call the "Shell Mechanism" and the feasibility of modifications on the DNA structure. The functionalities of the DNA shell, along with the enzyme that is captured, may allow us to open up fields of applications in chemical and medical applications, such as in bioreactors and in biomolecule detection. </p>
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<p style="font-size: 100%; font-weight: bold; margin-left: auto; margin-right: auto; text-align: center">fig2. Schematic animation of Medical DNA Shell system</p>
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<p style="font-size: 100%; font-weight: bold; margin-left: auto; margin-right: auto; text-align: center">Fig2. Schematic animation of Medical DNA Shell system</p>
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       <p>Medical functions such as detection of diseases with small amount of blood are possible by using microfluidics. For example, detecting and sensing the concentration of Thrombin which causes blood coagulation is possible by using Shell and microfluidics developed by our team. In the future, we will develop medical DNA Shell system for monitoring components of blood, detecting diseases, inputting medicine by DNA Shell, computing system integrated in microfluidics.</p>
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       <p>The DNA shell is made of three domains of DNA origamis. The two domains would be used to capture enzymes, and the last domain would be used to attach to a solid surface as is shown in Fig.2.
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Our experiments were done using a certain protein called Streptavidin. To widen the types of enzymes that could be captured in the DNA Shell, there needs to be more work to be done to find different aptamers that bind specifically to certain enzymes. However, with such developments, we hope and strongly believe that this nano-device would give innovative boost to the enzyme application.
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Revision as of 00:25, 27 October 2012

The University of Tokyo


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Fig1. Schematic animation of DNA Shell

Our focus for BIOMOD is to utilize DNA to make a shell like structure which can capture molecules inside the body, as if a shellfish is capturing its prey. The device, named DNA Shell, can exhibit functionalities such as highly sensitive detection, substrate protection, and attachment to solid surfaces. All these new functionalities are the result of this new capturing mechanism that we call the "Shell Mechanism" and the feasibility of modifications on the DNA structure. The functionalities of the DNA shell, along with the enzyme that is captured, may allow us to open up fields of applications in chemical and medical applications, such as in bioreactors and in biomolecule detection.


Fig2. Schematic animation of Medical DNA Shell system

The DNA shell is made of three domains of DNA origamis. The two domains would be used to capture enzymes, and the last domain would be used to attach to a solid surface as is shown in Fig.2. Our experiments were done using a certain protein called Streptavidin. To widen the types of enzymes that could be captured in the DNA Shell, there needs to be more work to be done to find different aptamers that bind specifically to certain enzymes. However, with such developments, we hope and strongly believe that this nano-device would give innovative boost to the enzyme application.


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