Biomod/2012/UTokyo/UT-Hongo/Method: Difference between revisions

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=='''AFM'''==
=='''AFM'''==
AFM Principle
 
We used AFM (Atomic Force Microscopy) for observing shell shape which we made.
We used AFM (Atomic Force Microscopy) for observing shell shape which we made.
Principle AFM is composed of cantilever, laser, photodiode, and detector and feedback electronics. AFM uses forces between the tip and sample. When cantilever approaches to sample, it deflected and this deflection is measured by laser. Reflected laser from cantilever is changed and its change is detected by photodiode and analyzed by feedback electronics
 
Scanning Mode
'''Principle'''
AFM is composed of cantilever, laser, photodiode, and detector and feedback electronics. AFM uses forces between the tip and sample. When cantilever approaches to sample, it deflected and this deflection is measured by laser. Reflected laser from cantilever is changed and its change is detected by photodiode and analyzed by feedback electronics
'''Scanning Mode'''
There are 3 kinds of scanning mode. In this project, we used trapping mode for getting shell images.
''Trapping Mode''
 
''Contact Mode''
 
''Non-contact Mode''


=='''蛍光光度計'''==
=='''蛍光光度計'''==

Revision as of 09:55, 19 October 2012

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<li class="toppage"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo">Top</a></li> <li class="motives"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Intro">Motives</a></li> <!-- <li class="design"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Function">Design</a></li> --> <li class="result"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly">Design & Results</a> <ul class="submenu"> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Assembly_of_the_DNA_Shell">Assembly of the DNA Shell</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Capturing_ability">Capturing Ability</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Immobilizing_on_microfluidic_device">Immobilizing on microfluidic device</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Supporting_Enzyme">Supporting Enzyme</a></li> </ul> </li> <li class="method"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Method">Method</a></li> <li class="futurework"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/FutureWork">Progress & Beyond</a></li> <li class="team"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Team">Team</a></li> <li class="acknowledgement"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Acknowledgement">Acknowledgement</a></li> 

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AFM

We used AFM (Atomic Force Microscopy) for observing shell shape which we made.

Principle AFM is composed of cantilever, laser, photodiode, and detector and feedback electronics. AFM uses forces between the tip and sample. When cantilever approaches to sample, it deflected and this deflection is measured by laser. Reflected laser from cantilever is changed and its change is detected by photodiode and analyzed by feedback electronics Scanning Mode There are 3 kinds of scanning mode. In this project, we used trapping mode for getting shell images. Trapping Mode

Contact Mode

Non-contact Mode

蛍光光度計

F-2500形日立分光蛍光光度計 具体的な実験操作方法

吸光光度計

TMSPC-8 Tm Analysis System SHIMADZU CORPORATION GeneQuant Pro 具体的な実験操作方法

電気泳動

1.Google Docs 2.試薬 試薬の調整

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