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Cantilever isn't contact with the sample. The distance between cantilever and sample is a few nanometers.
Cantilever isn't contact with the sample. The distance between cantilever and sample is a few nanometers.


=='''蛍光光度計'''==
=='''Fluorophotometer'''==
F-2500形日立分光蛍光光度計
F-2500形日立分光蛍光光度計
具体的な実験操作方法
具体的な実験操作方法


=='''吸光光度計'''==
=='''Absorption Spectrophotometer'''==
TMSPC-8 Tm Analysis System  SHIMADZU CORPORATION  
TMSPC-8 Tm Analysis System  SHIMADZU CORPORATION  
GeneQuant Pro
GeneQuant Pro
具体的な実験操作方法
具体的な実験操作方法


=='''電気泳動'''==
=='''Electrophoresis'''==
'''1.Google Docs'''
'''1.Google Docs'''
'''2.試薬'''
'''2.試薬'''
試薬の調整
試薬の調整
<center>[[Image:Biomod-2012-UTokyo-UT-Hongo- | 350px]]
<center>[[Image:Biomod-2012-UTokyo-UT-Hongo- | 350px]]

Revision as of 10:14, 19 October 2012

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<li class="toppage"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo">Top</a></li> <li class="motives"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Intro">Motives</a></li> <!-- <li class="design"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Function">Design</a></li> --> <li class="result"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly">Design & Results</a> <ul class="submenu"> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Assembly_of_the_DNA_Shell">Assembly of the DNA Shell</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Capturing_ability">Capturing Ability</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Immobilizing_on_microfluidic_device">Immobilizing on microfluidic device</a></li> <li><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Assembly#Supporting_Enzyme">Supporting Enzyme</a></li> </ul> </li> <li class="method"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Method">Method</a></li> <li class="futurework"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/FutureWork">Progress & Beyond</a></li> <li class="team"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Team">Team</a></li> <li class="acknowledgement"><a href="/wiki/Biomod/2012/UTokyo/UT-Hongo/Acknowledgement">Acknowledgement</a></li> 

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AFM

We used AFM (Atomic Force Microscopy) for observing shell shape which we made.

Principle AFM is composed of cantilever, laser, photodiode, and detector and feedback electronics. AFM uses forces between the tip and sample. When cantilever approaches to sample, it deflected and this deflection is measured by laser. Reflected laser from cantilever is changed and its change is detected by photodiode and analyzed by feedback electronics Scanning Mode There are 3 kinds of scanning mode. In this project, we used trapping mode for getting shell images.

Trapping Mode Move cantilever near to its resonance frequency, oscillate it up and down by piezoelectric. The amplitude of cantilever oscillation is decreased by interaction forces between cantilever and sample. Tapping mode lessens the damage to sample. Static Mode Cantilever drags the surface of sample. There are lots of noise and drift as dragging so low stiffness cantilevers are mainly used. Non-contact Mode Cantilever isn't contact with the sample. The distance between cantilever and sample is a few nanometers.

Fluorophotometer

F-2500形日立分光蛍光光度計 具体的な実験操作方法

Absorption Spectrophotometer

TMSPC-8 Tm Analysis System SHIMADZU CORPORATION GeneQuant Pro 具体的な実験操作方法

Electrophoresis

1.Google Docs 2.試薬 試薬の調整

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