Abstract - Medical DNA Shell
Methodologies to apply DNA for building precisely controlled nanostructures have greatly developed over the recent years. Our focus for BIOMOD is to utilize DNA to make a shell like structure which can capture molecules inside the shell-like body, as if a shellfish is eating its prey. Entrapment of the substrate was detected by the numerous florescent molecules that are attached to the body. This research may become a foundation for using DNA to mimic catalytic activity of enzymes.
Medical functions such as detection of diseases with small amount of blood are possible by using microfluidics. For example, detecting and sensing the concentration of Thrombin which causes blood coagulation is possible by using Shell and microfluidics developed by our team. In the future, we will develop medical DNA Shell system for monitoring components of blood, detecting diseases, inputting medicine by DNA Shell, computing system integrated in microfluidics.
The great scope of our research is to make a DNA origami structure that has functionalities similar to that of enzymes... however, that is far too much for students to do in one summer. Therefore, we chose to narrow down to making a structure which can bind to certain molecules and capture it, just like enzymes makes specific bondings to make ES complex.
We designed the structure shell-shaped so that it can efficiently capture target molecule with a dramatic change in the fluorescence for easy detection. We actually created three shell-shaped structures: plane shell, shell with florescent molecules and quenching molecules attached, shell with florescent molecules, quenching molecules, and biotin attached.
In this section, we write about our experiment. First, we mixed M13 and staples, and check whether DNAorigami was hybridized as designed by agarose gel electrophoresis and atomic force microscope.
In order to "catch" some kind of a substrate, the bonding between the lid and the bottom must be flexible enough to bend. Before we catch the target molecule (Streptavidin) by the DNA Shell, we have to make it clear whether the DNA Shell can be closed or not.