Biomod/2012/UTokyo/UT-Komaba/Experiment/Compatibility with Bistable: Difference between revisions
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==Experiments== | |||
===September 19th=== | ===September 19th=== | ||
Revision as of 23:12, 27 October 2012
Experiments
September 19th
- Normal DNA Origami
We prepared two types of DNA origami solution. The purpose of the experiment was to compare the different results from the different concentrations of the solution. Both concentrations of staple strands were as high as we did in September 12th.
- DNA Origami with Enzymes
We use enzymes in the bistable system, therefore, we make sure that the enzymes do not destroy the DNA origami. We put nickase, polymerase and exnuclease with DNA origami and stored them at the working temperature of the bistable. We used the DNA origami solution made on September 19th, enzymes and some essential chemicals
- DNA Origami with BSA
We were also worried if BSA interrupt the AFM and we can not get clear images of DNA origami. Therefore, we prepared a mix for September 20th. We put DNA origami solution made on September 19th, BSA and some essential chemicals.
September 20th
We observed three types of DNA origami made on September 19th. The three types is normal DNA origami, DNA origami with BSA and DNA origami with enzymes.
- Normal DNA Origami
We observed the origami by AFM, but the structures of both DNA origami were destroyed as you can see from the picture below. We suspect that it is due to keeping the origami in the freezer over night.
- DNA Origami with BSA
We need BSA for the bistable sytem so that we had to make sure that we can see the DNA structure when there are BSA in the liquid of the origami. Before we observed the origami with BSA by AFM, we rinsed 5μL of the origami liquid with 40μL of buffer for 4 times. Therefore, as you can see from the picture below, BSA did not disturb the observation of AFM, we can get the clear picture of DNA structure.
- DNA Origami with Enzymes
In the bistable system, we use three kinds of enzymes so that we had to make sure that the enzymes do not destroy the origami. As the result, we could see the DNA structure clearly, which proved that the enzymes do not destroy the DNA origami. Moreover, we observed the well-done origami structure in this experiment.
