Biomod/2012/UTokyo/UT-Komaba/Experiment/DNA Tablet
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Concept
The last but the most important experiment is the experiment of the DNA tablet. We combine modified origami and bistable system so that we can realize the DNA tablet. The purpose of the experiment is to observe the surface of the tablet and make sure that the surface change when we put another kind of DNA.
Experiment
October 20th
We got the modified staples, so we remade the staple mix and tried observing DNA origami. We annealed them from 90°C to 20°C for 2800 seconds. However, DNA origami weren't observed clearly.
(µL)
| staple mix | M13 | Mg | Buffer(TAE) | mQ | |
|---|---|---|---|---|---|
| 10x | 8 | 3.33 | 0.25 | 1 | 6.42 |
| 15x | 12 | 5 | 0.25 | 2 | 0.75 |
Staple Mix : 1µL of each staples and 184µL of TE
However, DNA origami wasn't observed clearly.
October 22nd
We made modified origami in September 20th, but the origami was not structured. Therefore, we carefully made the modified staple mix again.
October 23rd
We made the modified origami again. We annealed these tubes from 90°C to 20°C for 2800 minutes.
(µL)
| staple | M13(42nM) | Mg | Buffer(TAE) | mQ | Total Amount | |
|---|---|---|---|---|---|---|
| 10x | 8 | 4.8 | 0.25 | 2 | 4.95 | 20 |
| 5x | 4 | 2.4 | 0.25 | 2 | 11.35 | 20 |
October 24th
We observed the modified origami by AFM at the Komaba Campus. However, we could not get good pictures of modified origami from any tubes. From the pictures, the origami seemed to not be structured.
