Biomod/2012/UTokyo/UT-Komaba/Progress: Difference between revisions

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We confirmed that DNA origami structures weren't destroyed by environment of the bistable system.
We confirmed that DNA origami structures weren't destroyed by environment of the bistable system.


===Modified DNA Origami===
===Modified DNA Origami===
[[Image:Biomod-2012-UTkyo-UTKomaba-AFM-mod origami.png|thumb|229px|AFM image of modified Origami]]
[[Image:Biomod-2012-UTkyo-UTKomaba-AFM-mod origami.png|thumb|229px|AFM image of modified Origami]]


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==Bistable System==
==Bistable System==
[[Image:Biomod_2012_Utokyo_UT-Komaba_bistable-result.png‎|thumb|700px|center|the result of bistable system experiment]]
[[Image:Biomod_2012_Utokyo_UT-Komaba_bistable-result.png‎|thumb|700px|center|the result of bistable system experiment]]


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==DNA Origami+Bistable System==
==DNA Origami+Bistable System==
==Conclusion==

Revision as of 09:36, 26 October 2012

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<div id="title"><img src="http://openwetware.org/images/4/47/Biomod_2012_UTokyo_UT-Komaba_Top.png" alt="DNA tablet" width="800" height="120" onClick="this.src='http://openwetware.org/images/7/7d/BIOMOD_2012_UTokyo_UT-Komaba_title-animation.gif'"/></div>

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 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba">Home</a></li>
 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Idea">Idea</a></li>
 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Simulation">Simulation</a></li>
 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Experiment">Experiment</a></li>
 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Progress">Progress</a></li>
 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Episode">Episode</a></li>
 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Team">Team</a></li>
 <li><a href="/wiki/Biomod/2012/UTokyo/UT-Komaba/Supplementary">Supplementary</a></li>

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Goals And Achievement

DNA Origami

Original DNA Origami

AFM image of origami with BSA
AFM image of origami with enzymes

We confirmed that DNA origami structures weren't destroyed by environment of the bistable system.


Modified DNA Origami

AFM image of modified Origami

AFM confirmed that the modified Origami with covers worked well (you can see lines in the Origami

Bistable System

the result of bistable system experiment

This graph shows the status of the bistable system with time. In this graph, we tried to switch the state twice. As you can see, the first trial succeeded in reversing the concentration of X and V, changing the state. However, the second trial failed. We need to do more experiments to solve this problem.

DNA Origami+Bistable System

Conclusion