Biomod/2012/UTokyo/UT-Komaba/Progress: Difference between revisions

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=Goals And Achievements=
=Progress=
 
[[image:Biomod_2012_UTokyo_UT-Komaba_achievement.png|center]]
[[image:Biomod_2012_UTokyo_UT-Komaba_achievement.png|center]]


==DNA Origami==
==DNA Origami==
Line 9: Line 11:
===Original DNA Origami===
===Original DNA Origami===


[[Image:BIOMOD-2012-UTkyo-UTKomaba-AFM-0920-origami15×withBSA.png|300px|thumb|AFM image of origami with BSA]]
We confirmed that DNA origami structures weren't destroyed in the environment of the bistable system.
[[Image:BIOMOD-2012-UTkyo-UTKomaba-AFM-0920-origami15×withenzymes.png|300px|thumb|AFM image of origami with enzymes]]
 
{| class="noborder" style="margin-left: auto; margin-right: auto;"
|[[Image:BIOMOD-2012-UTkyo-UTKomaba-AFM-0920-origami15×withBSA.png|350px|thumb|AFM Image of Origami with BSA]]
|[[Image:BIOMOD-2012-UTkyo-UTKomaba-AFM-0920-origami15×withenzymes.png|350px|thumb|AFM Image of Origami with Enzymes]]
|}
 
[[Biomod/2012/UTokyo/UT-Komaba/Experiment/Original_Origami#September_20th|More Information]]


We confirmed that DNA origami structures weren't destroyed by the environment of the bistable system.


===Modified DNA Origami===
===Modified DNA Origami===


[[Image:Biomod-2012-UTkyo-UTKomaba-AFM-mod origami.png|thumb|229px|AFM image of modified Origami]]
[[Image:Biomod-2012-UTkyo-UTKomaba-AFM-mod origami.png|thumb|250px|AFM Image of Modified Origami|left]]
<br/>
 
We designed modified origami which showed three lines on the surface when we put cover DNA, and as you can see the picture above, we suceeded.
Therefore, we could confirm that the modified origami with covers worked well.
 
[[Biomod/2012/UTokyo/UT-Komaba/Experiment/Modified_Origami#October_5th|More Information]]


AFM confirmed that the modified Origami with covers worked well (you can see lines on the origami)


==Bistable System==
==Bistable System==


[[Image:Biomod_2012_Utokyo_UT-Komaba_bistable-result.png‎|thumb|700px|center|the result of bistable system experiment]]
[[Image:Biomod_2012_Utokyo_UT-Komaba_bistable-result.png‎|thumb|700px|center|The Result of the Experiment of the Bistable System]]
 


This graph shows the status of the bistable system over time. In this experiment, we tried to switch the state twice.
This graph shows the status of the bistable system over time. In this experiment, we tried to switch the state twice.
As you can see, the first trial succeeded in reversing the concentration of X and V, changing the state. However, the second trial failed. We need to do more experiments to solve this problem.
As you can see, the first trial succeeded in reversing the concentration of X and V, changing the state. However, the second trial failed. We need to do more experiments to solve this problem.
[[Biomod/2012/UTokyo/UT-Komaba/Experiment/Indirect_Bistable#October_3rd|More Information]]


==DNA Tablet==
==DNA Tablet==


We designed the DNA tablet and observed the two pictures, "I love DNA", "Tablet Boy".
We designed the DNA tablet and observed two pictures using AFM.


[[Image:Biomod_2012_UTokyo_UT-Komaba_tablet-desing.png‎|center|480px|border|Design of DNA tablet]]
[[Image:Biomod_2012_UTokyo_UT-Komaba_tablet-desing.png‎|center|480px|border|Design of DNA tablet]]


Without Inputs(plain DNA tablet):
 
===DNA tablet without Cover DNA===


[[Image:BIOMOD-2012-UTokyo-UTKomaba-PlainTablet.jpeg|200px]]
[[Image:BIOMOD-2012-UTokyo-UTKomaba-PlainTablet.jpeg|200px]]


I love DNA:


[[Image:BIOMOD-2012-UTokyo-UTKomaba-ILoveDNA.png|600px]]
==="I♡DNA"===
 
[[Image:BIOMOD-2012-UTokyo-UTKomaba-ILoveDNA.png|600px|I♡DNA]]
 
Unfortunately this image is not clear, but you can see that there is "I", the love heart, the ring of "D", and the partial shape of "A".
 
 
==="Tablet Boy"===


However the image is not clear, but we're sure that there is "I", the love heart, the ring of "D", and part of the shape of "A".
[[Image:BIOMOD-2012-UTokyo-UTKomaba-TabletBoy.png|400px|Tablet Boy]]


Tablet Boy:
This image is not also clear. There seems a square and some parts of Tablet boy's body.


[[Image:BIOMOD-2012-UTokyo-UTKomaba-TabletBoy.png|400px]]
[[Biomod/2012/UTokyo/UT-Komaba/Experiment/DNA_Tablet#October_26th|More Information]]


Again the image is not clear but we can see the square and some parts of Tablet boy's body.


==Conclusion==
==Conclusion==
In the end, we succeeded one switch of the bistable system and the design and the observation of DNA tablet.
Although there are still some difficulties, we can soon realize the DNA tablet which shows one simple picture and change it once.
Moreover, we can control the bistable system properly by setting the best initial condition, and also we can introduce the trioscillator system, too in near future.
Also, it is not impossible to realize the tablet which shows more pictures and a movie.
Although we have to solve the problem that complex pictures can not be observed by AFM, the tablet remains a lot of possibility of improvements.
__NOEDITSECTION__

Latest revision as of 02:16, 28 October 2012

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Progress


DNA Origami

Original DNA Origami

We confirmed that DNA origami structures weren't destroyed in the environment of the bistable system.

AFM Image of Origami with BSA
AFM Image of Origami with Enzymes

More Information


Modified DNA Origami

AFM Image of Modified Origami


We designed modified origami which showed three lines on the surface when we put cover DNA, and as you can see the picture above, we suceeded. Therefore, we could confirm that the modified origami with covers worked well.

More Information


Bistable System

The Result of the Experiment of the Bistable System


This graph shows the status of the bistable system over time. In this experiment, we tried to switch the state twice. As you can see, the first trial succeeded in reversing the concentration of X and V, changing the state. However, the second trial failed. We need to do more experiments to solve this problem.

More Information


DNA Tablet

We designed the DNA tablet and observed two pictures using AFM.

Design of DNA tablet
Design of DNA tablet


DNA tablet without Cover DNA


"I♡DNA"

I♡DNA

Unfortunately this image is not clear, but you can see that there is "I", the love heart, the ring of "D", and the partial shape of "A".


"Tablet Boy"

Tablet Boy

This image is not also clear. There seems a square and some parts of Tablet boy's body.

More Information


Conclusion

In the end, we succeeded one switch of the bistable system and the design and the observation of DNA tablet. Although there are still some difficulties, we can soon realize the DNA tablet which shows one simple picture and change it once. Moreover, we can control the bistable system properly by setting the best initial condition, and also we can introduce the trioscillator system, too in near future. Also, it is not impossible to realize the tablet which shows more pictures and a movie. Although we have to solve the problem that complex pictures can not be observed by AFM, the tablet remains a lot of possibility of improvements.


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