Biomod/2013/Fukuoka: Difference between revisions
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=Introduction= | =Introduction= | ||
==Background== | ==Background== | ||
* | * There are many important objects which have rotating parts in nature, industries, and in our life. For example, we find rotation of the earth, flagellum movement of Euglena, turbine for power generations, motors of cars and locomotives, etc. Again, rotation is an important movement in many ways.(Figure 1) | ||
==Problems== | ==Problems== | ||
Revision as of 12:02, 26 October 2013
| Introduction | Results and Disscution | Approaches | Member | Sponsor | YouTube |
|---|
Abstract
- Recent years, the technology of DNA origami is attracting keen interest for the synthesis of various nanostructures. But there are only few example of DNA origami with a rotating part, which are found in nature or industries: flagellum movement of Eugelena, turbines, and motors. Another problem is that the creation of the macroscopic-scale device only with DNA origami, which works with the macroscopic output or input signals is difficult because the synthesis of DNA-origami in large amount costs a lot. Here, we fabricate the device which detects the flow of surrounding water by combining inorganic nanoporous substrate with nanoscale weathercock made of DNA origami. The DNA weathercock consist of a blade and shaft parts are attached with a fluorescence molecule. We mount the DNA weathercock into the nanopore of the inorganic nanoporous substrate of size-tunable anodizing alumina, so that the DNA weathercock can freely rotate. When we give a flow, the DNA weathercock turns to the direction of the flow. We observe the behavior of fluorescence molecule with a fluorescence microscope or even by our eye through a polarizers set on the device.
Introduction
Background
- There are many important objects which have rotating parts in nature, industries, and in our life. For example, we find rotation of the earth, flagellum movement of Euglena, turbine for power generations, motors of cars and locomotives, etc. Again, rotation is an important movement in many ways.(Figure 1)
Problems
- However, only a few examples of a DNA-origami system with a movable part in themselves have been reported so far.
- It is also a problem that the synthesis of DNA origami in a large amount is not so easy, the creation of the macroscopic-scale device only with DNA origami which output or input the macroscopic signals such as a mechanical one.
Approaches
- In this study, we tried to fabricate the device in which an inorganic porous substrate is combined with DNA origami. We synthesize the DNA-origami-based “DNA weathercock” which has a shaft, a blade, and a fluorescence molecule. We then attach the weathercock onto the inorganic porous substrate (anodizing alumina) with regularly arranged perpendicular pores, so that the weathercock can freely rotate. We incorporate fluorescence quencher molecules on to a part of the substrate, so that the device emits fluorescence only when the liquid on the substrate flows along a specific direction.
Results and Disscution
- Fig.1 shows the AFM image of the synthesized DNA-weathercock. We observe many small objects, while we also find some aggregated large objects. On the enlarged AFM image (Fig.2), the object with the shape similar to the DNA origami designed with caDNAno (Fig.3) was observed. The Fig.4 is the cross-sectional view for the green line on Fig.2. While the size of the designed object has the length of 28 nm, width of 20 nm and the height 17 nm, the observed image is mostly in the same size.
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fig.1 AFM image of the synthesized DNA-weathercock
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fig.2 the enlarged AFM image
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fig.3 designed with caDNAno
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fig.4 cross-sectional view for the green line on Fig.2
Member
Students
- Maika Kuroki
- Shinya Anraku
- Ryo Iwashita
Adviser
- Naoya Yamaguchi
Professor
- Hajime Mita
- Nobuyoshi Miyamoto
Sponsor
YouTube
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