Biomod/2013/HKBU/experiment

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   <div id="header">    	 
       <ul class="topnav">
           <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU">HOME</a></li>
           <li>
               <a href="http://openwetware.org/wiki/Biomod/2013/HKBU/introduction#">DESIGN</a>               
               <ul class="subnav">
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/introduction#Project_Title">Project Title</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/introduction#Project_Abstract">Project Abstract</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/introduction#Result">Result</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/introduction#Discussion">Discussion</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/introduction#Potential_Application">Potential Application</a></li>
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           <li>
               <a href="http://openwetware.org/wiki/Biomod/2013/HKBU/methodology#">METHODOLOGY</a>
               <ul class="subnav">
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/methodology#List_of_Materials">List of Materials</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/methodology#SEM_imaging">SEM imaging</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/methodology#AFM_imaging">AFM imaging</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/methodology#FM_imaging">FM imaging</a></li>
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           <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/experiment#">PROCESS</a>
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                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/experiment#LATEST_RESULT">Latest Result</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/experiment#OCTOBER_FULL_LOG">October Full Log</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/experiment#SEPTEMBER_FULL_LOG">September Full Log</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/experiment#AUGUST_FULL_LOG">August Full Log</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/experiment#JULY_FULL_LOG">July Full Log</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/experiment#JUNE_FULL_LOG">June Full Log</a></li>
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                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/Team#Team">Team</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/Team#Member">Member</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/Team#Supervisor">Supervisor</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/Team#Graduate_Mentor">Graduate Mentor</a></li>
                   <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/Team#Collaborator">Collaborator</a></li>
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           <li>
               <a href="http://openwetware.org/wiki/Biomod/2013/HKBU/approaches">SUPPLEMENTARY</a>
               
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           <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/reference#">REFERENCE</a></li>
           <li><a href="http://openwetware.org/wiki/Biomod/2013/HKBU/acknowledge#">ACKNOWLEDGE</a></li>
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LAB LOGS

LATEST RESULT

October 26th

We finished the construction of wiki. The last Sunday meeting would be held tomorrow (October 27th), and we would have rehearsal on the presentation next week before heading to Harvard. Hello Boston! :)

October 25th

We completed the t-shirt design. The contents of wiki were kept being uploaded.

October 24th

We completed the awesome 3-min video and uploaded onto YouTube.

October 21st

We recorded the audio track of the video and combined the animation with the video.

October 20th

We revised the flash animation and improved some parts of the video.

October 18th

We continued working on the animation of the academic part of the video, and we decided to modify some parts of the video this Sunday (October 20th).

October 16th

We continued and completed the recording of the story part of the YouTube video with the help of Michael Huang. After the recording, we discussed the academic part of the video. We expect to finish the academic part by Saturday and start the sound recording.

October 13th

We held the 6th meeting of the semester. In the meeting, we planned the outline and distributed everyone’s role in the presentation. We also conducted the first trial of video recording with the help of Michael Huang. We will continue the video recording next Wednesday (October 16th)

October 10th

We conducted AFM imaging with the help of Mr. Fang Yuqiang today.

Results of AFM imaging:

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<html><p align="center">DNA origami, size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Abeta fibril, size scale: 1.0µm X 1.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Abeta + Streptavidin + DNA + QD, size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

Clear Aβ fibrils were shown. In addition, we performed analysis to the images of atomic force microscope.

October 8th

We conducted FM imaging with the help of Ms. Ashley Ho today.

Results of FM imaging:

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<html><p align="center">Abeta + Streptavidin + DNA + QD (1), size scale: 0.14mm X 0.14mm</p></html>

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<html><p align="center">Abeta + Streptavidin + DNA + QD (2), size scale: 0.14mm X 0.14mm</p></html>

We analyzed the images of fluorescence microscopy, and the slides for AFM imaging were prepared.

October 7th

We contacted Michael Huang, a sophomore student major in Cinema and Television, to support the recording of the YouTube video.

DNA samples were prepared for FM imaging on Tuesday.

October 6th

We held the fifth meeting of the semester. During the meeting, we discussed the flow and technical problems of the YouTube video, the design of the T-shirt, the results of experiment last week. We decided to conduct experiments for one more week to obtain some more confident results, and we were going to start the first trial of video recording next Sunday.

October 5th

A 1.5% agarose gel electrophoresis was conducted. Photo was captured, and the target bands were excised and undergone gel extraction according to the protocol (see methodology). After gel extraction and purification, the concentration of DNA was determined by Nanodrop.

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Result of gel electrophoresis:

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Result (inverted color):

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October 4th

A new annealing process was conducted at 17:20 today according to the parameters shown in August 23rd.

October 3rd

We conducted FM imaging with the help of Ms. Ashley Ho today. Results of FM imaging:

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<html><p align="center">Abeta + QD, size scale: 0.14mm X 0.14mm</p></html>

Clear and bright fibril patterns of QD were obtained.

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<html><p align="center">Abeta + Streptavidin + DNA + QD, size scale: 0.14mm X 0.14mm</p></html>

Clear and bright fibril patterns of QD were observed, but less QD aligned. The pattern is assumed to be caused by repulsion effect between streptavidin molecules and between DNA origami structures, which need to be supported by evidence from AFM imaging.

Diagram of the hypothesis:

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Results of AFM imaging:

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<html><p align="center">Slide 2 (Quantum dots), size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Slide 3 (DNA origami), size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Slide 4 (Streptavidin), size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Slide 5 (Abeta fibrils), size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Slide 6 (Abeta + QD), size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Slide 12 (Abeta + Streptavidin + DNA + QD), size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Slide 12 (2) (Abeta + Streptavidin + DNA + QD), size scale: 5.0µm X 5.0µm, height scale: -6.0nm – 6.0nm</p></html>

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<html><p align="center">Slide 12 (3) (Abeta + Streptavidin + DNA + QD), size scale: 1.0µm X 1.0µm, height scale: -6.0nm – 6.0nm</p></html>

Clear fibrils and STV-DNA-QD-conjugated fibrils were captured. The patterns of both “Abeta-QD” and “Abeta-Streptavidin-DNA-QD” have been obtained. Clear decorations of "STV-DNA-QD" on the fibril can be observed, which can be confirmed by the FM imaging, and there were less particles decorated on the streptavidin-coated Abeta fibrils compared with the sample "Abeta-QD", which supports the assumption that when conjugating STV and DNA origami, repulsion between these molecules reduce the number of QD attaching to the fibril. The next AFM imaging is reserved next Thursday (October 10th).

October 2nd

Slides for AFM imaging were prepared.

Details of the slides:

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OCTOBER FULL LOG

HKBU 2013 Lab_Diary_Oct.docx <html> <a href="http://openwetware.org/images/d/de/HKBU_2013_Lab_Diary_Oct.docx">Download & Check</a> </html>

SEPTEMBER FULL LOG

HKBU 2013 Lab_Diary_Sep.docx <html> <a href="http://openwetware.org/images/a/a0/HKBU_2013_Lab_Diary_Sep.docx">Download & Check</a> </html>

AUGUST FULL LOG

HKBU 2013 Lab_Diary_Aug.docx <html> <a href="http://openwetware.org/images/c/c1/HKBU_2013_Lab_Diary_Aug.docx">Download & Check</a> </html>

JULY FULL LOG

HKBU 2013 Lab_Diary_Jul.docx <html> <a href="http://openwetware.org/images/c/c5/HKBU_2013_Lab_Diary_Jul.docx">Download & Check</a> </html>

JUNE FULL LOG

HKBU 2013 Lab_Diary_Jun.docx <html> <a href="http://openwetware.org/images/4/48/HKBU_2013_Lab_Diary_Jun.docx">Download & Check</a> </html>


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