Biomod/2013/Komaba/Experiment: Difference between revisions
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Line 43: | Line 43: | ||
</th></tr> | </th></tr> | ||
<tr> | <tr> | ||
<td>Mg<math>2+</math> | <td>'''Mg'''<math>'''2+'''</math> | ||
</td><td>12.5mM | </td><td>12.5mM | ||
</td><td>1.25μL | </td><td>1.25μL | ||
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</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>TAE10x | <td>'''TAE10x''' | ||
</td><td> | </td><td> | ||
</td><td>10μL | </td><td>10μL | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>M13 | <td>'''M13''' | ||
</td><td>2.5nM | </td><td>2.5nM | ||
</td><td>6.25μL | </td><td>6.25μL | ||
Line 60: | Line 60: | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>Staple Mix | <td>'''Staple Mix'''</td><td>25nM | ||
</td><td>25nM | |||
</td><td>10μL | </td><td>10μL | ||
</td><td>250nM | </td><td>250nM | ||
</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td>mQ | <td>'''mQ''' | ||
</td><td> | </td><td> | ||
</td><td>72.5μL | </td><td>72.5μL | ||
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</td></tr> | </td></tr> | ||
<tr> | <tr> | ||
<td> Total | <td> '''Total''' | ||
</td><td> | </td><td> | ||
</td><td> 100μL | </td><td> 100μL |
Revision as of 11:20, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix (Cylinder)
Amount | Stock | ||
---|---|---|---|
Staple | 2.5μL(each) | 50μM | |
TE buffer | 52.5μL | ||
Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder.
Cylinder(10 - equivalent)
Concentration | TAE10x | Amount | Stock | |
---|---|---|---|---|
Mg[math]\displaystyle{ '''2+''' }[/math] | 12.5mM | 1.25μL | 1M | |
TAE10x | 10μL | |||
M13 | 2.5nM | 6.25μL | 40nM | |
Staple Mix | 25nM | 10μL | 250nM | |
mQ | 72.5μL | |||
Total | 100μL |