Biomod/2013/Komaba/Experiment: Difference between revisions
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Revision as of 11:37, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix (Cylinder)
Amount | Stock | ||
---|---|---|---|
Staple | 2.5μL(each) | 50μM | |
TE buffer | 52.5μL | ||
Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder.
Cylinder(10 - equivalent)
Concentration | Amount | Stock | |
---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M |
TAE10x | 10μL | ||
M13 | 2.5nM | 6.25μL | 40nM |
Staple Mix | 25nM | 10μL | 250nM |
mQ | 72.5μL | ||
Total | 100μL |
Cylinder(15 - equivalent)
Concentration | Amount | Stock | |
---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M |
TAE10x | 10μL | ||
M13 | 2.5nM | 6.25μL | 40nM |
Staple Mix | 37.5nM | 15μL | 250nM |
mQ | 67.5μL | ||
Total | 100μL |
Staple mix (Ring)
Amount | Stock | ||
---|---|---|---|
Staple | 5μL(each) | 50μM | |
TE buffer | 100μL | ||
Total | 500μL |
Stock of the staples are 50μM.
There were 80 strands for the Ring.
Ring(10- equivalent)
Concentration | TAE10x | Amount | Stock | |
---|---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M | |
TAE10x | 10μL | |||
M13 | 5nM | 12.5μL | 40nM | |
Staple Mix | 50nM | 10μL | 500nM | |
mQ | 66.25μL | |||
Total | 100μL |