Biomod/2013/Komaba/Experiment: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 80: | Line 80: | ||
===Cylinder(15 - equivalent)=== | ===Cylinder(15 - equivalent)=== | ||
<table> | |||
<tr> | |||
<th> | |||
</th><th>Concentration</th><th>TAE10x | |||
</th><th>Amount | |||
</th><th>Stock | |||
</th></tr> | |||
<tr> | |||
<td>'''Mg2+''' | |||
</td><td>12.5mM | |||
</td><td>1.25μL | |||
</td><td>1M | |||
</td></tr> | |||
<tr> | |||
<td>'''TAE10x''' | |||
</td><td> | |||
</td><td>10μL | |||
</td></tr> | |||
<tr> | |||
<td>'''M13''' | |||
</td><td>2.5nM | |||
</td><td>6.25μL | |||
</td><td>40nM | |||
</td></tr> | |||
<tr> | |||
<td>'''Staple Mix''' | |||
</td><td>37.5nM | |||
</td><td>15μL | |||
</td><td>250nM | |||
</td></tr> | |||
<tr> | |||
<td>'''mQ''' | |||
</td><td> | |||
</td><td>67.5μL | |||
</td><td> | |||
</td></tr> | |||
<tr> | |||
<td> '''Total''' | |||
</td><td> | |||
</td><td> 100μL | |||
</td><td> | |||
</td></tr></table> | |||
<p> <br> | |||
</p> |
Revision as of 11:25, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix (Cylinder)
Amount | Stock | ||
---|---|---|---|
Staple | 2.5μL(each) | 50μM | |
TE buffer | 52.5μL | ||
Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder.
Cylinder(10 - equivalent)
Concentration | TAE10x | Amount | Stock | |
---|---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M | |
TAE10x | 10μL | |||
M13 | 2.5nM | 6.25μL | 40nM | |
Staple Mix | 25nM | 10μL | 250nM | |
mQ | 72.5μL | |||
Total | 100μL |
Cylinder(15 - equivalent)
Concentration | TAE10x | Amount | Stock | |
---|---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M | |
TAE10x | 10μL | |||
M13 | 2.5nM | 6.25μL | 40nM | |
Staple Mix | 37.5nM | 15μL | 250nM | |
mQ | 67.5μL | |||
Total | 100μL |