Biomod/2013/Komaba/Experiment: Difference between revisions
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Revision as of 12:38, 16 October 2013
Home,Project,Design,Experiment,Supplementation,Team,Sponsors
- This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.
Lab Notes
We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.
September 11th Cylinder and Ring(first ver.)
Staple mix (Cylinder)
Amount | Stock | ||
---|---|---|---|
Staple | 2.5μL(each) | 50μM | |
TE buffer | 52.5μL | ||
Total | 500μL |
Stock of the staples are 50μM.
There were 179 strands for the Cylinder.
Cylinder(10 - equivalent)
Concentration | Amount | Stock | |
---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M |
TAE10x | 10μL | ||
M13 | 2.5nM | 6.25μL | 40nM |
Staple Mix | 25nM | 10μL | 250nM |
mQ | 72.5μL | ||
Total | 100μL |
Cylinder(15 - equivalent)
Concentration | Amount | Stock | |
---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M |
TAE10x | 10μL | ||
M13 | 2.5nM | 6.25μL | 40nM |
Staple Mix | 37.5nM | 15μL | 250nM |
mQ | 67.5μL | ||
Total | 100μL |
Staple mix (Ring)
Amount | Stock | ||
---|---|---|---|
Staple | 5μL(each) | 50μM | |
TE buffer | 100μL | ||
Total | 500μL |
Stock of the staples are 50μM.
There were 80 strands for the Ring.
Ring(10- equivalent)
Concentration | Amount | Stock | |
---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M |
TAE10x | 10μL | ||
M13 | 5nM | 12.5μL | 40nM |
Staple Mix | 50nM | 10μL | 500nM |
mQ | 66.25μL | ||
Total | 100μL |
Ring(15 - equivalent)
Concentration | Amount | Stock | |
---|---|---|---|
Mg2+ | 12.5mM | 1.25μL | 1M |
TAE10x | 10μL | ||
M13 | 5nM | 12.5μL | 40nM |
Staple Mix | 75nM | 15μL | 500nM |
mQ | 61.25μL | ||
Total | 100μL |
September 13th Observing Cylinder and Ring(first ver.) by AFM
This is the image of the Ring( 15- equivalent).
Unfortunately, since the AFM was out of control for a while, we couldn't take all all the images of our samples which we made on September 11th.
There might have been artificial mistakes, we decided to make the staple mix from the first.
September 13th Agarose Gel Electrophoresis(Cylinder and Ring)
Since we failed observe our origami by AFM, we decided to try electrophoresis.
Preparing Gel
Concentration | Amount | |
---|---|---|
Agarose | 1.75%(wt/V) | 0.525g |
TBE10x | 1x | 3mL |
Mg2+ | 12.5mM | 375μL |
mQ | up to 30mL | |
Total | 30mL |
- Pour the gel into frames.