Biomod/2013/Komaba/Experiment

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• This content is under construction. We have already ordered DNAs of some parts and they will arrive soon. On arrival, we will conduct experiments.

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Lab Notes

We conducted a lot experiment at Fuji Laboratory, Komaba Research Campus.

09/17(Tue)

Protocol

• Electrophoresis (After gelification)
  • Gel condition was good.
    • The gel was made in last experiment
  • Preparing Samples (Each 10μL & 2μL Loading Buffer)
    • Cylinder 10eq.
    • Cylinder 15eq.
    • Ring 10eq.
    • Ring 15eq.
    • Cylinder and Staples
    • Ring and Staples
    • M13
  • Pipetted Samples into the gel
  • Poured TBE into a container and charged 50V

Notes

• Result Picture explanation: Samples order by below list from left to right
  • C-10
  • C-15
  • C&StMix
  • M13
  • R&StMix
  • R-15
  • C-10