Biomod/2013/North Carolina State University/Procedure: Difference between revisions

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Now that the particles are water soluble and the excess surfactant has been removed, add 5000 thiolated oligonucleotides per gold nanorod and 3000 thiolated oligonucleotides per quantum dot.  Both sets of particles should be kept at room temperature and mixed at approx 300 rpm throughout the next step.  To facilitate binding slowly bring the salt concentration of the gold rods up to .5M and up to .1M for the quantum dots.  This is to facilitate the DNA landing on the particles.  Be careful not to add the salt too quickly as it will cause aggregation.  It is recommended that the NaCl be added in thirty minute intervals in .015M increments.  </p>
Now that the particles are water soluble and the excess surfactant has been removed, add 5000 thiolated oligonucleotides per gold nanorod and 3000 thiolated oligonucleotides per quantum dot.  Both sets of particles should be kept at room temperature and mixed at approx 300 rpm throughout the next step.  To facilitate binding slowly bring the salt concentration of the gold rods up to .5M and up to .1M for the quantum dots.  This is to facilitate the DNA landing on the particles.  Be careful not to add the salt too quickly as it will cause aggregation.  It is recommended that the NaCl be added in thirty minute intervals in .015M increments.  </p>


<p style="text-align:center; font-sixe:13pt;">
<p style="text-align:center; font-size:13pt;">
Now the gold nanorods and quantum dots are ready to be added to the origami.  Addition of the gold nanorods, quantum dots, and edge staple strands followed by an anneal yeilds our heterodimer.
Now the gold nanorods and quantum dots are ready to be added to the origami.  Addition of the gold nanorods, quantum dots, and edge staple strands followed by an anneal yeilds our heterodimer.
</p>
</p>


<img style
<div style="float:center; width 700px; padding:4px; background-color:white;">
<img src="https://dl-web.dropbox.com/get/Photos/BIOMOD%20photos/Asymmetrical%20heterodimer.png?w=AADdHT110-LbFWO2JnM8MAGwPODjkAiy41qmx0IuiaL1Vw" width="700" height="500" border="0" alt="heterodimer"></img>
</div>
 
<h3> Electrospinning <h3>
 
<p style="text-align:center; font-sixe:13pt;">
Previous work conducted by Dr. Tracey of North Carolina State University demonstrated that gold nanorods suspended in a polymer aligned themselves parallel to the axis of the polymer fiber after undergoing electrospinning.  After constructing our heterodimers we suspend them in Polyethylene Gycol (PEO).  We then electrospin them to produce fibers of diameter 15 micrometers.  The heterodimers are aligned parallel to the axis of the fiber due to the electronic effects of the large voltage used to electrospin the polymer.  The double stranded DNA strands (kite-tails) orient the heterodimers head to tail due to the increased drag experienced while drawn out into a fiber.  This creates an ordered array within the polymer fibers.  Placing the polymer fibers parallel to each other creates a macroscopic array of these nanoscale heterodimers.  </p>




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Revision as of 21:25, 26 October 2013

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     <a href="http://openwetware.org/wiki/Biomod/2013/NCSU" target="_blank"><img src="http://wheatonbible.org/Content/10713/Icons/home-icon.png" width="45" height="45" border="0" alt="DNAbeans Home"></img></a>
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<div style="float:center; Text-align:center;"> 

  <h1> Procedure </h1>

</div> <div style="float:center; text-align:center;"> 

   <h2> Functionalizing the Gold Nanorods and Quantum Dots <h2>

</div>

<p style="text-align:center; font-size:13pt;"> Our first steps are to coat the gold nanorods and quantum dots with single stranded DNA in order to bind them to the origami. Our gold nanorods are orginally coated with a surfactant called CTAB to prevent aggregation while our Quantum Dots are suspended in Toulene. Mercaptoethanol is added to the quantum dots to make them water soluble then both the gold rods and the quantum dots are spun down and resuspended in water. Resuspend twice for good measure. </p>

<p style="text-align:center; font-size:13pt;"> Now that the particles are water soluble and the excess surfactant has been removed, add 5000 thiolated oligonucleotides per gold nanorod and 3000 thiolated oligonucleotides per quantum dot. Both sets of particles should be kept at room temperature and mixed at approx 300 rpm throughout the next step. To facilitate binding slowly bring the salt concentration of the gold rods up to .5M and up to .1M for the quantum dots. This is to facilitate the DNA landing on the particles. Be careful not to add the salt too quickly as it will cause aggregation. It is recommended that the NaCl be added in thirty minute intervals in .015M increments. </p>

<p style="text-align:center; font-size:13pt;"> Now the gold nanorods and quantum dots are ready to be added to the origami. Addition of the gold nanorods, quantum dots, and edge staple strands followed by an anneal yeilds our heterodimer. </p>

<div style="float:center; width 700px; padding:4px; background-color:white;"> <img src="https://dl-web.dropbox.com/get/Photos/BIOMOD%20photos/Asymmetrical%20heterodimer.png?w=AADdHT110-LbFWO2JnM8MAGwPODjkAiy41qmx0IuiaL1Vw" width="700" height="500" border="0" alt="heterodimer"></img> </div>

<h3> Electrospinning <h3>

<p style="text-align:center; font-sixe:13pt;"> Previous work conducted by Dr. Tracey of North Carolina State University demonstrated that gold nanorods suspended in a polymer aligned themselves parallel to the axis of the polymer fiber after undergoing electrospinning. After constructing our heterodimers we suspend them in Polyethylene Gycol (PEO). We then electrospin them to produce fibers of diameter 15 micrometers. The heterodimers are aligned parallel to the axis of the fiber due to the electronic effects of the large voltage used to electrospin the polymer. The double stranded DNA strands (kite-tails) orient the heterodimers head to tail due to the increased drag experienced while drawn out into a fiber. This creates an ordered array within the polymer fibers. Placing the polymer fibers parallel to each other creates a macroscopic array of these nanoscale heterodimers. </p>


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