Biomod/2013/North Carolina State University/Procedure

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     <h2> #Functionalizing the Gold Nanorods and Quantum Dots <h2>
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Now that the particles are water soluble and the excess surfactant has been removed, add 5000 thiolated oligonucleotides per gold nanorod and 3000 thiolated oligonucleotides per quantum dot.  Both sets of particles should be kept at room temperature and mixed at approx 300 rpm throughout the next step.  To facilitate binding slowly bring the salt concentration of the gold rods up to .5M and up to .1M for the quantum dots.  This is to facilitate the DNA landing on the particles.  Be careful not to add the salt too quickly as it will cause aggregation.  It is recommended that the NaCl be added in thirty minute intervals in .015M increments.  </p>
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Now the gold nanorods and quantum dots are ready to be added to the origami.  Addition of the gold nanorods, quantum dots, and edge staple strands followed by an anneal yeilds our heterodimer.
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Revision as of 23:13, 26 October 2013

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Procedure

Functionalizing the Gold Nanorods and Quantum Dots

Our first steps are to coat the gold nanorods and quantum dots with single stranded DNA in order to bind them to the origami. Our gold nanorods are orginally coated with a surfactant called CTAB to prevent aggregation while our Quantum Dots are suspended in Toulene. Mercaptoethanol is added to the quantum dots to make them water soluble then both the gold rods and the quantum dots are spun down and resuspended in water. Resuspend twice for good measure.

Now that the particles are water soluble and the excess surfactant has been removed, add 5000 thiolated oligonucleotides per gold nanorod and 3000 thiolated oligonucleotides per quantum dot. Both sets of particles should be kept at room temperature and mixed at approx 300 rpm throughout the next step. To facilitate binding slowly bring the salt concentration of the gold rods up to .5M and up to .1M for the quantum dots. This is to facilitate the DNA landing on the particles. Be careful not to add the salt too quickly as it will cause aggregation. It is recommended that the NaCl be added in thirty minute intervals in .015M increments.

Now the gold nanorods and quantum dots are ready to be added to the origami. Addition of the gold nanorods, quantum dots, and edge staple strands followed by an anneal yeilds our heterodimer.

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