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Our project aims
at the construction of chain of molecules-releasing system by the trigger DNA input. They are problems in previous DDS(Drag Delivery System) that the control of timing releases trigger is difficult and only constant output can get from one time input.< /br> |+|
Our project aims chain-releasing system in the of is .<br>
|-|So, we created 2 systems to overcome these problems. The one is that releases trigger DNA on a certain condition. The other one is that increasing output by chain reaction of destroying liposome which starts from release of trigger.< /br> |+|
|-|At the first system, we make alginate hydrogel membrane including trigger , then voluntary dissolution of hydrogel and release of trigger happens by increasing temperature. At the second system, we destroy liposome by hybridizing trigger DNA to the surface of liposome. The liposome which is destroyed by trigger includes new trigger DNA so released trigger destroys neighboring liposome as chain reaction.< /br> |+|
The releases trigger a certain condition . The release trigger . <br>
|-|By creating these systems, it is expectable that creation of DDS which can control the timing of input and get a lot of output with one time input as application.< /br> |+|
the , trigger and temperature. the , the . is by trigger . <br>
a of .<br>
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Revision as of 06:06, 31 August 2013
Biomod2013 Sendai ver2.0
Our project aims to construct a chain-reactive molecule-releasing system in a spontaneous manner. Delivering drugs at appropriate places and controlling the quantity of released drug is very important to develop effective DDS. Our system makes these possible.
The system consists of two sub-systems: “egg-type initiator” and “chain-reactive burst”.
The “egg-type initiator” releases trigger DNAs under a certain condition like warming up to body temperature. The trigger DNAs hybridize to aptamer DNAs on liposomes, and the hybridization causes liposomes collapse. The collapsed liposomes release drugs and new trigger stored inside. These processes are the “chain-reactive burst”, and achieve sequential and exponential release of drugs.
To realize the egg-type initiator, trigger DNAs and chelate compounds are encapsulated in temperature-sensitive liposomes, and the liposomes are encapsulated in alginate hydrogel. Increasing temperature disrupts the liposomes, and then chelate compounds inside the liposomes melt the gels. Collapse of liposomes is realized by rapid deformation of liposomes mediated by hybridization between trigger and aptamer DNAs.
Our system will propose a new place and quantity controlling system of drug release by liposome-based drug delivery systems.