Recently genomics and proteomics have well studied, and new drugs such as siRNA have been developed. As a further effective approach to cure diseases, drug delivery systems (DDS) in living bodies have been studied. Liposome and polymers are usually used in DDS as containers.


Controlling the quantity of molecular release is very important to develop effective release system.
Delivering drugs molecular at appropriate places which are usually very narrow spaces is another important point to avoid adverse effect.
If initial releasing signals are released at a very limited place, and if the signals continuously transduce the initial signals with releasing molecular, are the two important points fulfilled? This is the motivation of our project.

Project Goal

Our project aims to construct a chain-reactive molecule-releasing system in a spontaneous manner like organisms.
If chain-reactive collapse of liposomes happens in a limited space, release of drugs inside liposomes is expected to be controlled around a limited space. Our chain-reactive burst system is a good one for doing this strategy.
Each liposome encapsulates trigger DNAs and ペイロード, and has aptamer DNAs in their outer surface.ペイロードとはある個所で必要とされる物質のことで、例えば薬や化学的なシグナルとなる物質が挙げられる。
Hybridizations of trigger DNAs and aptamer DNAs deform liposome rapidly, and consequently, the liposomes are destroyed. This destruction continuously occurs by releasing new trigger DNAs inside liposomes to disrupt neighbor liposomes. These processes achieve concentrated drug release. We call these processes as “Chain-reactive burst.”



To develop the egg-type initiator, following experiments are needed. We plan to create the egg-type initiator by combining these sub-parts.

シグナルを感知するシステムを備えたチェーンリアクティブバーストを実現するために以下の実験が必要である。 (1) トリガーDNAとリポソームを破壊するアプタマーDNAの設計と構築 (2) 周辺のリポソームの破壊によって放出された新しいトリガーによりリポソームの破壊の連鎖が起こることの確認 (3) 温度上昇を感知して割れるリポソームの機能確認 To develop the chain-reactive burst, following experiments are needed.
(1) Design and construction of trigger DNA and aptamer DNA to cause collapse of liposomes.
(2) Confirm chain reaction of liposomal collapse by new triggers inside disrupt neighbor liposomes.
(3) Collapse of liposomes by temperature shift