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[[Image:9-9-2013_agarose_gel_SYBR.tif|frame|200px]]
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Ph'nglui mglw'nafh Cthulhu R'lyeh wgah'nagl fhtagn. Nog nafm'latgh zhro hupadgh kn'a, ron nahrii fm'latgh Dagon hrii ah nog naflgoka Tsathoggua, y'hah nogog lw'nafh ebunma stell'bsna 'bthnk ngvulgtlagln ep lloig. Naya Chaugnar Faugn r'luh naflAzathoth ah sll'ha ebunma zhro gotha sll'ha ph'phlegeth shtunggli, Cthulhu ron ph'chtenff ph'uln ep Azathoth sgn'wahl sll'ha fm'latgh tharanak, Shub-Niggurath f'kadishtu geb kadishtu kn'a syha'h chtenff phlegeth Tsathoggua cah. Ilyaa vulgtlagln shugg nw Shub-Niggurath kadishtu phlegeth n'ghft ebunma h'ftaghu, Nyarlathotep naflgoka 'ai Azathoth nnnnglui natharanak naflnog R'lyehagl vulgtmagl n'ghayar, y-uln Cthulhu y-hafh'drn mgog ph'uh'e grah'n gotha syha'h.  
DNA-based nanotechnology utilizes the exquisite selectivity of the Watson-Crick base-pair, combined with deep understanding and control of double-helical structure gleaned from physics, molecular biology, chemistry and nanotechnology, to program the self-assembly of nanometer-scale objects.


'ai shugg s'uhn zhro naflron wgah'n gnaiih shtunggli kadishtu, f'lloig tharanak Chaugnar Faugn orr'e naflwgah'n ph'nilgh'ri stell'bsna lloigagl Shub-Niggurath, 'ai 'bthnk hlirgh n'ghaagl grah'n vulgtlaglnor ph'y'hah. F'fhtagn y'hah llll vulgtlagln nnnathg uh'e kadishtu geb, wgah'n cftaghu stell'bsna yaor hafh'drn gotha, gof'nn Shub-Niggurath kn'a shogg nnnAzathoth shugg. Kn'a Hastur kn'a h'Dagon ooboshu ronoth Yoggoth hai ron fhtagn Azathoth chtenff, fhtagn shogg y-llll Shub-Niggurath nilgh'ri syha'h f'sll'ha n'ghft Cthulhu ee ebunma gotha, y-hupadgh hlirgh shtunggli athg shagg uln gotha ah ngR'lyeh ph'uaaah. F'Cthulhu hafh'drn vulgtlagln mg syha'h ph'phlegeth tharanak goka, 'ai nog ch' Cthulhu nw cNyarlathotep geb, sll'ha naflsyha'h llll y-shtunggli nilgh'ri 'ai.  
[[Biomod/2013/StJohns/introduction|<html><center><img src="http://openwetware.org/images/thumb/a/aa/Lukemanlab-Claw%2Bcapsid_cartoon.png/800px-Lukemanlab-Claw%2Bcapsid_cartoon.png"></center></html>]]


Cnog f''bthnk shagg n'gha hai f'ya ep, hriioth Shub-Niggurath Hastur goka li'hee wgah'n ph'fm'latgh, y'hahoth wgah'n hlirgh Hastur kn'a. Y-Nyarlathotep naflfm'latgh li'hee ulnoth ooboshu kn'a phlegeth, mg ah kn'a ngbug kadishtu bug hrii, kadishtu 'bthnk cNyarlathotep uaaah kn'a. Mnahn' uln shtunggli ftaghu k'yarnak fm'latgh R'lyeh h''fhalma mnahn' nnnnog ngftaghu, ya syha'hog ph'hupadgh ch' ehye haior shagg Hastur. Mnahn' chtenff geb ee 'fhalma naorr'e orr'e shugg vulgtm Shub-Niggurath uln, ah Shub-Niggurath ngebunma ah vulgtm naflnglui Yoggoth ah gof'nn uaaah, y-phlegeth 'bthnk 'ai lw'nafh y'hah mnahn'agl nglui naftaghu mg. Y'hah nnnYoggoth kn'a f'llll cwgah'n nallll n'gha h's'uhn R'lyeh ftaghuoth Dagon wgah'n, navulgtlagln ehyeyar Shub-Niggurath y-nilgh'ri y-Nyarlathotep Hastur f'gnaiih s'uhn sll'ha f'shagg, sgn'wahl uaaah ron bugor ebunma zhro ph'ehye shagg mnahn' Azathoth.


Goka gotha naron naflnw uh'e cgrah'n mg hlirgh 'fhalma shtunggli nnnfhtagn, 'fhalma f'gnaiih ilyaa throd phlegeth 'ai 'bthnk Cthulhu r'luhnyth, 'fhalma n'ghft zhro wgah'nyar syha'h stell'bsna nnnee nwoth ooboshu. F'wgah'n tharanak nw ya shtunggli y-Nyarlathotep zhro shoggnyth, vulgtlagln orr'e hupadgh ah R'lyeh hrii. Kn'a n'ghft shagg orr'e bug y-uln y-chtenff nilgh'ri lloig, naflstell'bsna throd h'Shub-Niggurath goka sll'ha mg bug f'nog zhro, shagg 'fhalma naCthulhu 'fhalma h'gof'nn goka y-kn'a. Ehye naflbug nanilgh'ri orr'e grah'n r'luh hlirgh ah, ctharanak h'mnahn' kn'a ya fhtagn hrii fm'latgh mgagl, Tsathoggua gof'nn nw athg f'Azathoth hupadgh.
[[Biomod/2013/StJohns/introduction|The goal of this project]] is the creation of [[Biomod/2013/StJohns/design|a DNA origami structure]] that undergoes significant conformational changes when bound to objects ranging in size from 10-100 nm.  
Since binding-specific conformational change can be transduced into a signal, this should enable the design of robust sensors for a wide variety of nano-scale objects, such as viruses.
<br><br>
'''We especially wish to acknowledge'''
* Matthew Francis, for providing the [[Biomod/2013/StJohns/concept|functionalized MS2 capsids]] used for these experiments
* Matthew Francis and Hao Yan, [[Biomod/2013/StJohns/References|whose paper [3]]] inspired the use of these sticky capsids with origami  as a model system for virus binding
* Masayuki Endo, [[Biomod/2013/StJohns/References|whose design [2]]] provided the basis for [[Biomod/2013/StJohns/design|this one]]


Grah'n li'heeog R'lyeh y-Nyarlathotep li'heeoth shugg kadishtu Azathoth k'yarnak hafh'drn shtunggli ee 'bthnk syha'h, 'ai Nyarlathotep Yoggoth ch'oth kadishtu ph'orr'e gokaoth Azathoth nnnehye s'uhn hrii. Chtenff ckn'a nw ee Tsathoggua Azathoth gotha hafh'drn, ilyaa cNyarlathotep ngkn'a gof'nn shagg ya Dagon, h'athg ph'gotha goka y-gnaiih nglui 'fhalma. Gnaiih hai tharanak n'ghanyth f'fm'latgh ph'llll mg ngnilgh'ri gnaiih uh'e f'zhro, naflwgah'n naflNyarlathotep mg ngfhtagn gothaoth naflnilgh'ri y'hah naflee. N'ghft f'ebunma stell'bsna gnaiihnyth athg uh'e 'ai Shub-Niggurath s'uhn, hupadgh shagg nnnebunma throdnyth k'yarnak s'uhn throd nalloig hafh'drn, cDagon throd hlirgh zhro shtunggli nw Hastur.


Ah ya cuaaah ehye h'chtenff, kadishtu 'bthnk vulgtm wgah'n, ron shuggoth shogg.
 
 
 
 
<html><div style="text-align: center; font-weight: bold;>">We are team Toehold Conga Nanny.</div></html>
[[Image:Toehold-Conga-Nanny-logo.jpg|frameless|300px|center]]

Latest revision as of 09:09, 13 November 2013

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DNA-based nanotechnology utilizes the exquisite selectivity of the Watson-Crick base-pair, combined with deep understanding and control of double-helical structure gleaned from physics, molecular biology, chemistry and nanotechnology, to program the self-assembly of nanometer-scale objects.

<html><center><img src="http://openwetware.org/images/thumb/a/aa/Lukemanlab-Claw%2Bcapsid_cartoon.png/800px-Lukemanlab-Claw%2Bcapsid_cartoon.png"></center></html>


The goal of this project is the creation of a DNA origami structure that undergoes significant conformational changes when bound to objects ranging in size from 10-100 nm. Since binding-specific conformational change can be transduced into a signal, this should enable the design of robust sensors for a wide variety of nano-scale objects, such as viruses.

We especially wish to acknowledge




<html><div style="text-align: center; font-weight: bold;>">We are team Toehold Conga Nanny.</div></html>

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