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The primary structure under investigation is a DNA origami 'claw' consisting of three rigid 'prongs' joined by three flexible linkages. This structure is composed of one long circular 'backbone' strand of ssDNA (m13p18 plasmid) held in shape by many short linear 'staple' strands. The overall structure can be diagrammed in two dimensions: | The primary structure under investigation is a DNA origami 'claw' consisting of three rigid 'prongs' joined by three flexible linkages. This structure is composed of one long circular 'backbone' strand of ssDNA (m13p18 plasmid) held in shape by many short linear 'staple' strands. The overall structure can be diagrammed in two dimensions: | ||
[[Image:Lukemanlab-2013-0016.png|frameless|border|600px]] | |||
[[Image:Lukemanlab-clawdesign.png|frameless|border|600px]] | [[Image:Lukemanlab-clawdesign.png|frameless|border|600px]] | ||
Revision as of 12:13, 26 October 2013
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Claw
The primary structure under investigation is a DNA origami 'claw' consisting of three rigid 'prongs' joined by three flexible linkages. This structure is composed of one long circular 'backbone' strand of ssDNA (m13p18 plasmid) held in shape by many short linear 'staple' strands. The overall structure can be diagrammed in two dimensions:
For FRET analysis, we add fluorescent dyes to the ends of some of the 'staple' strands. By varying the strand to which these dyes are added, we can change the location and orientation of the tags on the macrostructure.
Yan/Francis Triangle
This DO triangle[3] presents a surface area similar to that of the claw and can be functionalized with single-stranded binding elements. It differs from the claw in that it is rigid, so binding with the substrate should not cause a change in conformation.
Sherman 90-Degree Triangle
These DO triangles are the basic design being used for our experiments with controlling hinge angles in DNA origami.
