Biomod/2013/Tianjin: Difference between revisions

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<a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Designs/Self-assemblyTrack">Self-assembly Track</a>
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<a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Designs/DeliveryDevice">The Delivery Device</a>
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<a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments &amp; Results">Polymerizing</a>
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<a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments &amp; Results/Cleavage">Cleavage</a>
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<a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments &amp; Results/DeliveryDevice">Delivery Device</a>
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= Abstract =
<html>
<p>As we all know that there are several tapes of DNA-walkers, which belongs to a specific kind of DNA nano-robot. This kind of device has a very bright future in molecular transport, but sometimes it’s not that convenient to build a special track for the particular walker, especially in vivo transportation. So, compact with the transportation that really happened in human body, we hope to design a delivery system that can perform an automatic transport, and we really made efforts to it this summer vacation.</p>
<p>We came across a method called HCR (Hybridization Chain Reaction) last year. This method was used as an amplifier in detection of some specific molecular. We try to use this method to construct a track, and make this track could be induced and self-assembled. We designed 4 DNA strands numbered T1 to T4, which could be induced to perform a polymerize reaction and form a track for the DNA-walker to walk. These 4 strands each has 2 stem-loop structures, so that they will be stable enough to keep no polymerize reaction without the inducer. We can also make a terminator for this track. The terminate DNA will stop the DNA from polymerizing. This kind of track forming technique makes DNA-walker a better potential in transportation in vivo or some other conditions that could be difficult to build DNA-tracks with planting or a huge annealing.</p>
<p>As for the inducer, it’s just a short DNA strand, and we can call it trigger DNA. If it could be attached to a DNA-origami box full of workers, a delivery device is just in to use. The track will form along the trigger, and it will stop when meeting another DNA-origami box attached with the terminate DNA. Then, the walker could be released and transport the cargo between two DNA-origami boxes. These are just future fantasies, we’ve not been gone that far, but we really think the self-assembly track as an amazing tool.</p>


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<p><br />
<p><br /><br />
<br />
E-mail：austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code：300072
E-mail：austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code：300072
<br />Copyright 2013 © Tianjin University iGEM Team</p>
<br />Copyright 2013 © Tianjin University Biomod Team</p>
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<a href="http://openwetware.org/index.php?title=Biomod/2013/Tianjin&action=edit">edit</a>
 




Latest revision as of 21:14, 27 October 2013

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               <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Designs">Background</a>

<a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Designs/Self-assemblyTrack">Self-assembly Track</a> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Designs/DeliveryDevice">The Delivery Device</a> </div> </li> <li><a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments &amp; Results" onmouseover="mopen('m4')" onmouseout="mclosetime()" style="height:35px;padding:8px 0px 7px 0px;">Experiments &amp; Results</a> 

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<a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments &amp; Results">Polymerizing</a> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments &amp; Results/Cleavage">Cleavage</a> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments &amp; Results/DeliveryDevice">Delivery Device</a>

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<div class="fixed"> <div> <br/> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Team"><img src="http://openwetware.org/images/5/58/TJU-TEAM.jpg" width="280px"></a> </div> <br/> <div> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Designs"><img src="http://openwetware.org/images/6/69/TJU-DESIGNS.jpg" width="280px"></a> </div> <br/> <div> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Experiments_%26_Results"><img src="http://openwetware.org/images/8/8d/TJU-EX%26RE.jpg" width="280px"></a> </div> <br/> <div> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Protocol"><img src="http://openwetware.org/images/d/d6/TJU-PROTOCOL.jpg" width="280px"></a> </div> <br/> <div> <a href="http://openwetware.org/wiki/Biomod/2013/Tianjin/Attributions"><img src="http://openwetware.org/images/0/0b/TJU-ATTRIBUTIONS.jpg" width="280px"></a> </div>

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Abstract

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<p>As we all know that there are several tapes of DNA-walkers, which belongs to a specific kind of DNA nano-robot. This kind of device has a very bright future in molecular transport, but sometimes it’s not that convenient to build a special track for the particular walker, especially in vivo transportation. So, compact with the transportation that really happened in human body, we hope to design a delivery system that can perform an automatic transport, and we really made efforts to it this summer vacation.</p> <p>We came across a method called HCR (Hybridization Chain Reaction) last year. This method was used as an amplifier in detection of some specific molecular. We try to use this method to construct a track, and make this track could be induced and self-assembled. We designed 4 DNA strands numbered T1 to T4, which could be induced to perform a polymerize reaction and form a track for the DNA-walker to walk. These 4 strands each has 2 stem-loop structures, so that they will be stable enough to keep no polymerize reaction without the inducer. We can also make a terminator for this track. The terminate DNA will stop the DNA from polymerizing. This kind of track forming technique makes DNA-walker a better potential in transportation in vivo or some other conditions that could be difficult to build DNA-tracks with planting or a huge annealing.</p> <p>As for the inducer, it’s just a short DNA strand, and we can call it trigger DNA. If it could be attached to a DNA-origami box full of workers, a delivery device is just in to use. The track will form along the trigger, and it will stop when meeting another DNA-origami box attached with the terminate DNA. Then, the walker could be released and transport the cargo between two DNA-origami boxes. These are just future fantasies, we’ve not been gone that far, but we really think the self-assembly track as an amazing tool.</p>


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<div class="link"> <p><br /><br /> E-mail：austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code：300072 <br />Copyright 2013 © Tianjin University Biomod Team</p> </div>





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