Biomod/2013/Waterloo

Video

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Required Materials

1. 1M Tris-HCl, pH 7.5
2. 0.5M Acetic Acid
3. 100mM EDTA
4. 100mM Magnesium Acetate
5. DNA Working Stocks (~1uM): Walker-{1-7}
6. 1x 200uL PCR tube

Procedure

1. Remove DNA working stocks from the freezer and allow them to thaw
2. Prepare the buffer:
3. Add 49.5 uL of milli-Q water to the PCR tube
4. Add 12.5 uL (6+6.5uL) of 100mM Magnesium Acetate to the PCR tube
5. Add 4 uL of 1M Tris-HCl to the PCR tube
6. Add 4 uL of 500 mM Acetic Acid to the PCR tube
7. Add 2.5 uL of 100 mM EDTA to the PCR tube
8. Vortex the PCR tube for 30 seconds