Biomod/2013/Waterloo

Video

Kristopher hurry up.

Required Materials

1. 1M Tris-HCl, pH 7.5
2. 0.5M Acetic Acid
3. 100mM EDTA
4. 100mM Magnesium Acetate
5. DNA Working Stocks (~1uM): Walker-{1-7}
6. 1x 200uL PCR tube

Procedure

1. Remove DNA working stocks from the freezer and allow them to thaw
2. Prepare the buffer:
3. Add 49.5 uL of milli-Q water to the PCR tube
4. Add 12.5 uL (6+6.5uL) of 100mM Magnesium Acetate to the PCR tube
5. Add 4 uL of 1M Tris-HCl to the PCR tube
6. Add 4 uL of 500 mM Acetic Acid to the PCR tube
7. Add 2.5 uL of 100 mM EDTA to the PCR tube
8. Vortex the PCR tube for 30 seconds

Determine A260 of each strand using the spectrophotometer using Oligocalc (http://www.basic.northwestern.edu/biotools/OligoCalc.html) and the sequence of the strand, determine the concentration of the strand