Biomod/2014/Fukuoka: Difference between revisions

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<img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">


<hr>
<br>


<div id="header"><h1><p style="background:#CCFFFF; color:purple;"><font face=cursive size="6"><B>  Team FIT </font> </B></p></a></h1></div>
         


<ul id="dropmenu">
<table> <tr align="center">
<td cellspacing="10" cellpadding="10" width="1055" width="180" height="60" bgcolor="#BAD3FF"><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka"><B><font face=cursive color="#003366" size="3">Top</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Introduction.html"><B><font face=cursive color="#003366" size="3">Introduction</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Approach and Goals.html"><B><font face=cursive  color="#003366" size="3" >Approach and Goals</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Method.html"><B><font face=cursive color="#003366" size="3">Method</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1300" width="180" bgcolor="#BAD3FF"><a href="fit_Results and Discussion.html"><B><font face=cursive color="#003366" size="3">Results and Discussion</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Member.html"><B><font face=cursive color="#003366" size="3">Member</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Sponsor.html"><B><font face=cursive color="#003366" size="3">Sponsor</font></B></a></td>    </tr></table>


  <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
</div>
<h2 p style="background:#BAD3FF; color:#003366;"><font face=cursive size="5"><B> Abstract  </font></B></p></h2>
<h3>我々は DNA の相補的塩基対形成を利用して「ナノ黒ひげ危機一髪」を作成する。黒ひげ危
機一髪は、人形が入った樽の特定箇所に剣を刺したときに人形が離れて飛んでいく玩具で
ある。まず樽部分の DNA を、ミスマッチを含む相補 DNA の人形と結合させておく。そこ
に樽部分 DNA と完全相補となる剣 DNA を加えると、樽と剣が相補鎖を形成し頭部が離脱
する。DNA おりがみ技術でこれらを作成することも検討したが、観察面、コスト面から現
実的ではない。そこで安価に合成可能でかつ観察が容易な、DNA 修飾シリカ粒子を利用す
る。これまでに、ゾルゲル反応によるメソポーラスシリカ球の合成と表面への DNA 修飾を
行い、また蛍光分子修飾 DNA を用いて相補鎖形成の確認をした。本研究は、特定の DNA
分子情報に応答する物質の選択的放出制御装置と見なすも事もでき、ドラッグデリバリー
システムや分子情報増幅などへの応用も期待できる。</h3>


    <ul>


      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#abs">Abstract</a></li>
      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#vid">Video</a></li>
 
    </ul>
  </li>
  <li><a href="fit_Introduction.html#pro">Projects</a>
    <ul>
      <li><a href="fit_Introduction.html#back">Background & Motivation</a></li>
      <li><a href="fit_Introduction.html#goal">Project Goals</a></li>


    </ul>
  </li>
  <li><a href="fit_Approach and Goals.html#des">Design</a>
    <ul>
      <li><a href="fit_Approach and Goals.html#ear">Early Design</a></li>
      <li><a href="fit_Approach and Goals.html#fin">Final Design</a></li>
     
    </ul>
  </li>
  <li><a href="fit_Method.html#met">Method</a>
    <ul>
      <li><a href="fit_Method.html#a">Preliminary Experiment</a></li>
      <li><a href="fit_Method.html#b">Synthesis of the “Barrel” particles and the “Doll” particles</a></li>
      <li><a href="fit_Method.html#c">Combining the Doll particles with the Barrels particles</a></li>
　　　<li><a href="fit_Method.html#d">Pop-up of the doll particle</a></li>
　　　<li><a href="fit_Method.html#e">Materials</a></li>
  </ul>
  </li>
  <li><a href="fit_Results and Discussion.html#">Result and Discussions</a>
    <ul>
      <li><a href="fit_Results and Discussion.html#a">Preliminary Experiment</a></li>
      <li><a href="fit_Results and Discussion.html#b">Synthesis of the “Barrel” particles and the “Doll” particles</a></li>
      <li><a href="fit_Results and Discussion.html#c">Pop-up of the doll particle</a></li>
      <li><a href="fit_Results and Discussion.html#d">Conclusions</a></li>
　    </ul>
  </li>
  <li><a href="fit_Member.html#team">Team</a>
    <ul>
      <li><a href="fit_Member.html#men">Member</a></li>
      <li><a href="fit_Member.html#spo">Sponsor</a></li>
     
    </ul>
  </li>
</ul>




</div>
</body>
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<a name="abs"></a>
<div align="left"  style="margin: 1px 60px;">
<center><font size="6" color="#000022" face="Arial"><b>Abstract</b></font></center>


<font size="4" color="#000022" face="Arial">


image figure...
<p>
&nbsp;  Pop-up pirate is a famous Japanese toy. When players stab a "sword" to the specific point on a "barrel", a "doll" pops up from the "barrel". Here we fabricate this toy on nanoscale.We first synthesize the silica particles attached with ssDNA: the “Barrel particle”. We then synthesize "doll particle" attached with complementary ssDNA with partial mismatches. We combine these particles by hybridization to form doll-barrel pairs. If we add completely complementary “sword ssDNA”, the "doll" will be replaced by "sword ssDNA" and will "pop up". We introduce fluorescence molecules on the ssDNAs, so that we can observe the pop-up event by fluorescence microscope and can visualize the sword-barrel hybridization by detecting FRET. Furthermore, we use mesoporous silica as the particle which can containsubstances in the pores. Since the release of the colloid particles and substances are triggered by molecular stimuli, this system will be applicable for DDS etc. <br>




[[Image:Dnakurohige.gif|320px|thumb|center|fig.3-1]]
</p>


<hr>


<br>
　</p>


<a name="vid"></a>
<center><font size="6" color="#000022" face="Arial"><b>Video</b></font></centar>
<hr>
</div>
</font>
<br>
<hr />


<br><center><!--<youtube>O8wbCKPCjko</youtube>-->
<iframe width="640" height="360" src="//www.youtube.com/embed/O8wbCKPCjko?feature=player_detailpage" frameborder="0" allowfullscreen></iframe></center>


<br><br><br>
</div>


 
</body>
<hr />

Latest revision as of 20:59, 25 October 2014

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<img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">



  • <a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
  • <a href="fit_Introduction.html#pro">Projects</a>
    • <a href="fit_Introduction.html#back">Background & Motivation</a>
    • <a href="fit_Introduction.html#goal">Project Goals</a>
  • <a href="fit_Approach and Goals.html#des">Design</a>
    • <a href="fit_Approach and Goals.html#ear">Early Design</a>
    • <a href="fit_Approach and Goals.html#fin">Final Design</a>
  • <a href="fit_Method.html#met">Method</a>
    • <a href="fit_Method.html#a">Preliminary Experiment</a>
    • <a href="fit_Method.html#b">Synthesis of the “Barrel” particles and the “Doll” particles</a>
    • <a href="fit_Method.html#c">Combining the Doll particles with the Barrels particles</a>
      • 　　　
    • <a href="fit_Method.html#d">Pop-up of the doll particle</a>
      • 　　　
    • <a href="fit_Method.html#e">Materials</a>
  • <a href="fit_Results and Discussion.html#">Result and Discussions</a>
    • <a href="fit_Results and Discussion.html#a">Preliminary Experiment</a>
    • <a href="fit_Results and Discussion.html#b">Synthesis of the “Barrel” particles and the “Doll” particles</a>
    • <a href="fit_Results and Discussion.html#c">Pop-up of the doll particle</a>
    • <a href="fit_Results and Discussion.html#d">Conclusions</a>
      • 　
  • <a href="fit_Member.html#team">Team</a>
    • <a href="fit_Member.html#men">Member</a>
    • <a href="fit_Member.html#spo">Sponsor</a>


<a name="abs"></a>

Abstract

  Pop-up pirate is a famous Japanese toy. When players stab a "sword" to the specific point on a "barrel", a "doll" pops up from the "barrel". Here we fabricate this toy on nanoscale.We first synthesize the silica particles attached with ssDNA: the “Barrel particle”. We then synthesize "doll particle" attached with complementary ssDNA with partial mismatches. We combine these particles by hybridization to form doll-barrel pairs. If we add completely complementary “sword ssDNA”, the "doll" will be replaced by "sword ssDNA" and will "pop up". We introduce fluorescence molecules on the ssDNAs, so that we can observe the pop-up event by fluorescence microscope and can visualize the sword-barrel hybridization by detecting FRET. Furthermore, we use mesoporous silica as the particle which can containsubstances in the pores. Since the release of the colloid particles and substances are triggered by molecular stimuli, this system will be applicable for DDS etc.


　

<a name="vid"></a>

Video</centar>



<iframe width="640" height="360" src="//www.youtube.com/embed/O8wbCKPCjko?feature=player_detailpage" frameborder="0" allowfullscreen></iframe>




</body>

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