Biomod/2014/Hokudai/DESIGN: Difference between revisions
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<p class="clearLeft | <p class="clearLeft">(Figure1: Formation of well-oriented microtubules with preferential polarity polymerized under temperature gradient.)</p> | ||
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<p class="clearLeft example6">The well-oriented microtubules will start to move after adding multimeric kinesin * and ATP(Figure2). </p> | <p class="clearLeft example6">The well-oriented microtubules will start to move after adding multimeric kinesin * and ATP(Figure2). </p> | ||
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<p class="clearLeft | <p class="clearLeft">(Figure2: Uniaxial contraction of well-oriented microtubules by adding multimeric kinesin at the nucleation site of microtubules.)</p> | ||
<p class="example6"><br>*Kinesins were assembled into multi-motor clusters by crosslinking of non-motor domain of kinesin. This mimics the functions of myosin filaments of the muscle.</br></p> | <p class="example6"><br>*Kinesins were assembled into multi-motor clusters by crosslinking of non-motor domain of kinesin. This mimics the functions of myosin filaments of the muscle.</br></p> | ||
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<p class=" | <p class="clearLeft">(Figure 3: Schematic diagram of the microtubule nucleation site by light irradiation and an artificial muscle having sarcomere.)</br></p> | ||
Revision as of 07:23, 29 August 2014
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<p class="example7">1) Control of the contraction direction with well-oriented microtubules</p> <p class="example6">Microtubule will be polymerized from tubulin monomer using IR light irradiation in a confined cell with a temperature gradient. Under this temperature gradient, the nucleus is formed at the warm end, and the growth process progresses toward the cold end. When the density or length of the filaments exceeds a critical value, well-oriented microtubules with preferential polarity will be obtained (Figure1).</p>
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<p><a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/4/48/%E5%BE%AE%E5%B0%8F%E7%AE%A1%E3%81%AE%E9%85%8D%E5%90%91%28%E8%8B%B1%E8%AA%9E%29.png" vspace="10" hspace="10" width="450" height="225" border="0" /></a> <a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/f/f2/DESIGN%28Figure3%29.JPG" vspace="10" hspace="10" width="450" height="350" border="0" /></a> </p> </div>
<p class="clearLeft">(Figure1: Formation of well-oriented microtubules with preferential polarity polymerized under temperature gradient.)</p>
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<p class="clearLeft example6">The well-oriented microtubules will start to move after adding multimeric kinesin * and ATP(Figure2). </p>
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<p><a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/d/dc/DESIGN02%28last%29.JPG" vspace="10" hspace="10" width="450" height="350" border="0" /></a> <a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/7/70/%E5%8F%8E%E7%B8%AE.gif" vspace="10" hspace="10" width="450" height="350" border="0" /></a> </p> </div>
<p class="clearLeft">(Figure2: Uniaxial contraction of well-oriented microtubules by adding multimeric kinesin at the nucleation site of microtubules.)</p> <p class="example6"><br>*Kinesins were assembled into multi-motor clusters by crosslinking of non-motor domain of kinesin. This mimics the functions of myosin filaments of the muscle.</br></p> </br> <p class="example7 clearLeft"> 2) Formation of the artificial sarcomere of muscle by photo irradiation</p> <p class="example6">We can form multiple microtubule nucleation sites with temperature gradient created by photo irradiation. After adding kinesin and ATP, it will work as artificial sarcomere(Figure3).</p>
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<p><a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/6/6f/DESIGN%28Figure4%29.jpg" vspace="10" hspace="10" width="450" height="350" border="0" /></a> <a class="image" title="team hokudai"><img alt="team hokudai" src="https://upload.wikimedia.org/wikipedia/commons/d/d5/DESIGN%28Figure4%29last.JPG" vspace="10" hspace="10" width="450" height="350" border="0" /></a> </p> </div> <p class="clearLeft">(Figure 3: Schematic diagram of the microtubule nucleation site by light irradiation and an artificial muscle having sarcomere.)</br></p>
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