Biomod/2014/Kansai/Results

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Design

Experiment and Result

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Problem & Solution

We had difficulty to coupling dumbbell hairpins on the direction orthogonal to the origami. It was solved to use 3 sheets of DNA origami.

Aspect ratio of 1 dot

Height And Width	: 2 DNA 3nm×2=6 nm
Width	: 2 pitch 3.4nm×2=6.8 nm

To construct DNA nano QR code uses 3 types of DNA origami. Normally, they have required 600 pieces of DNA staple (200 pieces per one DNA origami).We devised the sequences of Staple DNA, they were shared among 3 types of DNA origami. thus they required only 359 pieces of DNA staple.

Design

　　　　　QR code　　　　　　　　　　　　DNA Origami sequence
　　　　　　　　　　　　　　　　　　　 　(This is part of the design)

　　　　　　　　　　　　　　　　　　　black points are dumbbell hairpin

We designed one kind of DNA nano QR code.
A black part on QR code is expressed by dumbbell hairpin.
The DNA nano QR code is a secret until we create it at nano size.

This is a design of joint points between part A and part B.
Sequences indicated red lines connect part A and part B.
they also exist between part B and part C.
So, the structure is constructed to connect three kinds of part.