Biomod/2014/Kashiwa/Experiments: Difference between revisions
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<p class="image"><img src=""></p> | <p class="image"><img src=""></p> | ||
<p class="caption">Fig.1-1-1. TEM image of the Wall. | <p class="caption">Fig.1-1-1. TEM image of the Wall. | ||
</div> | |||
<p class="paragraph">In this experiment, the assembly condition of the Wall structure was optimized and results were analyzed by agarose gel electrophoresis. | <p class="paragraph">In this experiment, the assembly condition of the Wall structure was optimized and results were analyzed by agarose gel electrophoresis. | ||
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The folding is corroborated by the TEM image. | The folding is corroborated by the TEM image. | ||
</p> | </p> | ||
<br clear="right"> | |||
<h1 class="big">1-1(b). Producing MISTIC</h1> | <h1 class="big">1-1(b). Producing MISTIC</h1> |
Revision as of 11:45, 24 October 2014
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<ul id="menu"> <font face="Frutiger,Helvetica,Arial"> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa"><img src="http://openwetware.org/images/e/ec/LogoKashiwa.png" onmouseover="this.src='http://openwetware.org/images/7/7a/Logo2Kashiwa.png'" onclick="this.src='http://openwetware.org/images/1/1d/Logo2.5.png'" onmouseout="this.src='http://openwetware.org/images/e/ec/LogoKashiwa.png'" height="80px" width="120px" name="def"></a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Project" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">PROJECT</span></a> <ul class="sub"> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Project#1" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Background</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Project#2" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Motivation</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Project#3" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Project Goals</span></a></li> </ul> </li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Trial" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;"> EARLY TRIAL </span></a> <ul class="sub"> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Trial#1" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Design</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Trial#2" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Approaches</span></a></li> </ul> </li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Design" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">DESIGN</span></a> <ul class="sub"> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Design#2" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">The Receptor</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Design#1" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">The Motor</span></a></li> </ul> </li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Highlights" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;"> EXPERIMENT </span></a> <ul class="sub"> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Highlights" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Highlights</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Receptor" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">The Receptor</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Motor" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">The Motor</span></a></li> </ul> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Discussion" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;"> DISCUSSION </span></a> <ul class="sub"> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Discussion#1" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Achievements</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Discussion#2" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Future</span></a></li> </ul> </li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Protocols" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">PROTOCOL</span></a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Team" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">TEAM</span></a> <ul class="sub"> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Team#1" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Members</span></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2014/Kashiwa/Team#2" onMouseOver="On('img1')" onMouseOut="Off()"><span style="font-size:12pt;">Sponsors</span></a></li> </ul> </li> </font> </ul>
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<body> <a name="1"></a> <font face="Futura,Arial,Frutiger" font size="24px">EXPERIMENTS</font> <br> <br>
<h1 class="title"><a name="background"> Highlights</a></h1> <br>
<div class="imagebox">
<p class="image"><img src="http://openwetware.org/images/9/96/Koala_005.jpg" width="300" height="213"></p> <p class="caption">It's the caption.<br>The agarose gel electrophoresis.</p>
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<div class="CollapsibleBox" id="CBoxCover1">
<div class="CollapsibleBoxTitle"> <p class="cBoxButtons"> <a href="#" onclick="HideCBox('CBoxBody1'); return false;" title="折りたたみ/復元"><font color="white" font size="2">[show/hide]</font></a> </p> <p class="cBoxTitle"> Contents:</p> </div> <div class="CollapsibleBoxBody" id="CBoxBody1"> <ul style="list-style:none;"> <li> <a href=#1> 1. The Sensing System: The Receptor </a> <ul style="list-style:none;"> <li> <a href="#1-1">1-1. Preparing the components </a></li> <ul style="list-style:none;"> <li> <a href="#1-1-1">1-1(a). Folding the Wall </a></li> <li> <a href="#1-1-2">1-1(b). Producing MISTIC </a></li> <li> <a href="#1-1-3">1-1(c). Designing the Activator </a></li> </ul> </li> <li> <a href="#1-2">1-2. Embedding the Wall into the liposome </a></li> <li> <a href="#1-3">1-3. Linking the Activator to the liposome </a></li> <li> <a href="#1-4">1-4. Combining the Wall and the Activator </a></li> <li> <a href="#1-5">1-5. Separating the Wall from the Activator </a></li> </ul> </li> <li> <a href="#2">2. The Moving System: The Motor </a> <ul style="list-style:none"> <li> <a href="#2-1">2-1. Producing the Motor-Monomer </a></li> <li> <a href="#2-2">2-2. Deactivating and activating the binding capacity of streptavidin </a></li> <li> <a href="#2-3">2-3. Putting the Motor-Monomers into the liposome </a></li> </ul> </li> </ul> </div>
</div>
<a name="2"></a> <br> <h1 class="title"><a name="background"> The Receptor</a></h1>
<h1 class="sub">1-1. Preparing the components </h1>
<h1 class="big">1-1(a). Folding the Wall</h1>
<div style="imagebox">
<p class="image"><img src=""></p> <p class="caption">Fig.1-1-1. TEM image of the Wall.
</div>
<p class="paragraph">In this experiment, the assembly condition of the Wall structure was optimized and results were analyzed by agarose gel electrophoresis. </p>
<p class="paragraph">The optimum results were following: <ul style="list-style-type:disc; list-image-type:disc;">
<li>Concentration of MgCl2 is 15 mM.</li> <li>Temperature of annealing is 45.3 ℃.</li> <li>Time of annealing is 5 hours.</li> <li>Concentration of NaCl is 2.5 mM.</li>
</ul> </p>
<p class="paragraph"> The folding is corroborated by the TEM image. </p>
<br clear="right">
<h1 class="big">1-1(b). Producing MISTIC</h1>
<h1 class="big">1-1(c). Designing the Activator</h1>
<h1 class="sub">1-2. Embedding the Wall into the liposome</h1>
<h1 class="sub">1-3. Linking the Activator to the liposome</h1>
<h1 class="sub">1-4. Combining the Wall and the Activator</h1>
<hi class="sub">1-5. Separating the Wall from the Activator</h1>
<a name="3"></a> <br> <br> <br> <h1 class="title"><a name="background"> The Motor</a></h1>
<h1 class="sub">2-1. Producing the Motor-Monomer </h1>
<h1 class="sub">2-2. Deactivating and activating the binding capacity of streptavidin </h1>
<h1 class="sub">2-3. Putting the Motor-Monomers into the liposome </h1> <br>
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