Biomod/2014/OhioMOD: Difference between revisions

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<h2>Abstract (not final)</h2>
<h2>Abstract (not final)</h2>
<p2>MicroRNA are 19-25 nucleotide, epigenetic regulatory RNA which are attractive therapeutic targets for several reasons. Their structure immediately suggests that antisense nucleotides can be used to modify their expression. They are also known regulators of a wide variety of cellular activities, including apoptosis and cell cycle control. Here, we report the creation of DNA nanostructures incorporating overhangs capable of binding and sequestering miR-21 in OSU-CLL cells. First, we describe how the structures were designed, synthesized, and validated. We use TEM images and agarose gel electrophoresis to confirm the shape of the structures and show that our structures can sequester miR-21 using Typhoon gel imaging. Next, we confirm that our nanostructures are uptaken in OSU-CLL cells via endocytosis by showing that our structures are co-localized with lysosomes in cells. Finally, we study the viability and reproduction of OSU-CLL cells after exposure to our nanostructures. <i>The structures are shown by cell counting and flow cytometry to induce apoptosis in 150% of OSU-CLL cells. We also measure expression of PTEN, a tumor suppressor which is repressed by miR-21 to elucidate a mechanism of action of our nanostructures. We show that PTEN levels in OSU-CLL incubated with our structures are significantly increased. </i></p2>
<p2>MicroRNA are 19-25 nucleotide, epigenetic regulatory RNA which are attractive therapeutic targets for several reasons. Their structure immediately suggests that antisense nucleotides can be used to modify their expression. They are also known regulators of a wide variety of cellular activities, including apoptosis and cell cycle control. Here, we report the creation of DNA nanostructures incorporating overhangs capable of binding and sequestering miR-21 in OSU-CLL cells. First, we describe how the structures were designed, synthesized, and validated. We use TEM images and agarose gel electrophoresis to confirm the shape of the structures and show that our structures can sequester miR-21 using Typhoon gel imaging. Next, we confirm that our nanostructures are uptaken in OSU-CLL cells via endocytosis by showing that our structures are co-localized with lysosomes in cells. Finally, we study the viability and reproduction of OSU-CLL cells after exposure to our nanostructures. <i>The structures are shown by cell counting and flow cytometry to induce apoptosis in 150% of OSU-CLL cells. We also measure expression of PTEN, a tumor suppressor which is repressed by miR-21 to elucidate a mechanism of action of our nanostructures. We show that PTEN levels in OSU-CLL incubated with our structures are significantly increased. </i></p2>
<h3>Video</h3>
<h1>Video</h1>
<p3>The 2014 video is coming soon. Until then, enjoy OhioMOD's videos from the past two years!</p3>
<p3>The 2014 video is coming soon. Until then, enjoy OhioMOD's videos from the past two years!</p3><br>
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<div id="video">

Revision as of 11:19, 21 August 2014

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     <li ><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD">Home</a></li>
     <li><a href="http://openwetware.org/wiki/Biomod/2014/OhioMOD/project">Background</a></li>

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</style> <!--css stuff ends here--> </head> <body> <div id="logo"> <img src="http://openwetware.org/images/e/ed/OHIOMODLOGO2.png" alt="OhioMOD"> <iframe src="//www.facebook.com/plugins/like.php?href=https%3A%2F%2Fwww.facebook.com%2FOhioMod&amp;width&amp;layout=standard&amp;action=like&amp;show_faces=true&amp;share=true&amp;height=80" scrolling="no" frameborder="0" style="border:none; overflow:hidden; height:80px;" allowTransparency="true"></iframe> </div> <div id="abstract"> <h1>Welcome to OhioMOD</h1> <p1>OhioMOD is Ohio State's undergraduate biomolecular design team. The team participates in the BIOMOD international competition each year at Harvard University. The 2014 project will use a folded DNA structure to control miRNA gene regulation in cancer.</p1> <h2>Abstract (not final)</h2> <p2>MicroRNA are 19-25 nucleotide, epigenetic regulatory RNA which are attractive therapeutic targets for several reasons. Their structure immediately suggests that antisense nucleotides can be used to modify their expression. They are also known regulators of a wide variety of cellular activities, including apoptosis and cell cycle control. Here, we report the creation of DNA nanostructures incorporating overhangs capable of binding and sequestering miR-21 in OSU-CLL cells. First, we describe how the structures were designed, synthesized, and validated. We use TEM images and agarose gel electrophoresis to confirm the shape of the structures and show that our structures can sequester miR-21 using Typhoon gel imaging. Next, we confirm that our nanostructures are uptaken in OSU-CLL cells via endocytosis by showing that our structures are co-localized with lysosomes in cells. Finally, we study the viability and reproduction of OSU-CLL cells after exposure to our nanostructures. <i>The structures are shown by cell counting and flow cytometry to induce apoptosis in 150% of OSU-CLL cells. We also measure expression of PTEN, a tumor suppressor which is repressed by miR-21 to elucidate a mechanism of action of our nanostructures. We show that PTEN levels in OSU-CLL incubated with our structures are significantly increased. </i></p2> <h1>Video</h1> <p3>The 2014 video is coming soon. Until then, enjoy OhioMOD's videos from the past two years!</p3><br> </div> <div id="video"> <iframe width="560" height="315" src="//www.youtube.com/embed/d1NVwkxnS-s" frameborder="0" allowfullscreen></iframe> <iframe width="560" height="315" src="//www.youtube.com/embed/_RK5GbcpEqQ" frameborder="0" allowfullscreen></iframe>


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