|
|
| (One intermediate revision by one other user not shown) |
| Line 1: |
Line 1: |
| {{Biomod/2014/Sendai/temp/0821/Styles}}
| |
| <html>
| |
| <head>
| |
| <title></title>
| |
| </head>
| |
|
| |
|
| <body>
| |
| <div id="header">
| |
| <div id="homebutton">
| |
| <li id="stamp"><a href="http://openwetware.org/wiki/Biomod/2014/Sendai">Home</a></li>
| |
| </div>
| |
|
| |
|
| |
| <div id="globalnav" class="dis">
| |
| <ul>
| |
| <li id="gn-home"><a href="/wiki/Biomod/2014/Sendai">Home</a></li>
| |
| <li id="gn-intro"><a href="/wiki/Biomod/2014/Sendai/Introduction">Introduction</a></li>
| |
| <li id="gn-design"><a href="/wiki/Biomod/2014/Sendai/Design">Design</a></li>
| |
| <li id="gn-simu"><a href="/wiki/Biomod/2014/Sendai/Simulation">Simulation</a></li>
| |
| <li id="gn-xp"><a href="/wiki/Biomod/2014/Sendai/Experiment">Experiment</a></li>
| |
| <!--<li id="gn-protocol"><a href="/wiki/Biomod/2014/Sendai/Protocol">Protocol</a></li>-->
| |
| <li id="gn-dis"><a href="/wiki/Biomod/2014/Sendai/Discussion">Discussion</a></li>
| |
| <li id="gn-team"><a href="/wiki/Biomod/2014/Sendai/Team">Team</a></li>
| |
| <li id="gn-end"><a href="#"></a></li>
| |
| </ul>
| |
| </div>
| |
| </div>
| |
| <div id="main">
| |
| <h1>Conclusion&Discussion</h1>
| |
| <section>
| |
| <p>
| |
| In our project, we proposed a system that releases molecules following the
| |
| information written on DNA. The features of this system are programmability and
| |
| scalability. To achieve our goals, we created two types of system, Enzyme system and
| |
| Enzyme-free system. In both of these systems, programmability and scalability are
| |
| confirmed through simulations. Results of the simulation are shown below.
| |
| Both Enzyme and Enzyme-free systems have a common limitation, which is that the same
| |
| output cannot be released again. In concrete terms, input coded A-B-A is not accepted
| |
| while input coded A-B-C is accepted.
| |
| The reaction rate constant is planned to be optimized by feed-backing the result of
| |
| chemical reaction.
| |
| (1) Enzyme system
| |
| First we verified the programmability of Enzyme system. When information of input
| |
| was a-b, output A-B was released in order. The same thing applies to input coded b-
| |
| a. We can conclude that Enzyme system has programmability. By adjusting initial
| |
| concentration, the time interval of the output was changed.
| |
| However, we found it difficult to adjust the time interval of the output arbitrarily. We
| |
| are searching for solution for this problem.
| |
| The simulation in which information of input was a-b-c to show the scalability is
| |
| now proceeding by extending ordinary differential equations.
| |
| (2) Enzyme-free system
| |
| In the same manner as Enzyme system, we carried out the simulation of Enzyme-
| |
| free system. When information of input was a-b, output A-B was released in order.
| |
| The same result of the simulation was obtained when information of input was b-a as
| |
| well. Subsequently, we simulated the scalability. When information of the input was a-
| |
| b-c, output A-B-C was released. We can conclude that Enzyme-free system has both
| |
| programmability and scalability.
| |
| We found that a slight difference in the concentrations of components has a
| |
| great effect on the intervals of output releasing, which makes it difficult to tune the
| |
| whole reactions in detail.
| |
| We have to make experiments to confirm that the two systems have
| |
| programmability, and Enzyme-free system has scalability. We have already finished
| |
| designing the sequence of DNA. We have prepared all the materials and are about
| |
| to go on experiments to prove these features.
| |
| </p>
| |
| </section>
| |
| </div>
| |
| <div style="background-color:#5b2d01; margin-top:30px; padding-top:15px; width:100% ;height:100px; color:#fff; font-size: 125%; position: absolute; bottom: 0; display: inline;">
| |
| <div style="float:left; height:auto; width:30%; padding-left:20%; ">
| |
| (C)Copyright Biomod 2014 Team Sendai<br />
| |
| E-MAIL:<a href="mailto:teamsendai2014@gmail.com"><span style="color:white">teamsendai2014@gmail.com</span></a>
| |
| </div>
| |
| <div style="height:auto; width:30%; padding-left:20%; float:left">
| |
| <a href="http://www.molbot.mech.tohoku.ac.jp/index.html">
| |
| <img src="http://openwetware.org/images/f/f3/Muratalab-icon2_dark-01.png" width="200" alt="Molcular Robotics Lab"></a>
| |
| </div>
| |
| </div>
| |
| </body>
| |
| </html>
| |
