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<img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">
<hr>
<br>


<div id="header"><h1><p style="background:#CCFFFF; color:purple;"><font face=cursive size="6"><B>  Team FIT </font> </B></p></a></h1></div>
         


<ul id="dropmenu">
<table> <tr align="center">
<td cellspacing="10" cellpadding="10" width="1055" width="180" height="60" bgcolor="#BAD3FF"><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka"  link="#003366" alink="FFFF66" vlink="#003366"><B><font face=cursive color="#003366" size="3">Top</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Introduction.html"><B><font face=cursive color="#003366" size="3">Introduction</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Approach and Goals.html"><B><font face=cursive  color="#003366" size="3" >Approach and Goals</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Method.html"><B><font face=cursive color="#003366" size="3">Method</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1300" width="180" bgcolor="#BAD3FF"><a href="fit_Results and Discussion.html"><B><font face=cursive color="#003366" size="3">Results and Discussion</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Member.html"><B><font face=cursive color="#003366" size="3">Member</font></B></a></td>
<td cellspacing="10" cellpadding="10" width="1055" width="180" bgcolor="#BAD3FF"><a href="fit_Sponsor.html"><B><font face=cursive color="#003366" size="3">Sponsor</font></B></a></td>    </tr></table>


  <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
</div>
<h2 p style="background:#BAD3FF; color:#003366;"><font face=cursive size="5"><B> Introduction </font></B></p></h2>
<div style="padding: 35px;">
<h3>  <p> &nbsp; DNAは生体高分子の一つであり、近年このDNAを素材として高次元構造をつくるDNAナノテクノロジーが注目を集めている。塩基配列を自在に設計することで、制御可能なナノ構造と機能材料として期待されている。中でもドラッグデリバリーシステム（DDS）はDNA origamiでつくられた立方体の中に薬剤を入れ、患部に直接薬剤を投与できることで医療の分野で注目されている。（ドラッグデリバリーの画像）<br>
　しかしながら、既存のドラッグでは一つの容器に一つの薬剤しか入れることができない。またDNAを大量合成することは容易ではないこと、放出した薬剤を目視では観察することができないことが課題である。<br>
　そこで吸着剤としてよく用いられるメソポーラスシリカに着目した。メソポーラスシリカは孔の大きな制御することができ、またシリカは人体にも含まれていることから、人体への影響がないこと、表面に化学修飾ができることから、メソポーラスシリカの孔に薬剤を入れることでドラッグデリバリーとして用いることができないかと考えた。また、放出する制御をDNAによってできないかと考えた。メソポーラスに複数の薬剤を充填することで、患者の症状に合わせて薬剤を選択でき、また複数の薬剤を組み合わせて治療を行うことが期待できる。
</p>
</h3>


    <ul>


      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#abs">Abstract</a></li>
      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#vid">Video</a></li>
 
    </ul>
  </li>
  <li><a href="fit_Introduction.html#pro">Projects</a>
    <ul>
      <li><a href="fit_Introduction.html#back">Background & Motivation</a></li>
      <li><a href="fit_Introduction.html#goal">Project Goals</a></li>


    </ul>
  </li>
  <li><a href="fit_Approach and Goals.html#des">Design</a>
    <ul>
      <li><a href="fit_Approach and Goals.html#ear">Early Design</a></li>
      <li><a href="fit_Approach and Goals.html#fin">Final Design</a></li>
     
    </ul>
  </li>
  <li><a href="fit_Method.html#met">Method</a>
    <ul>
      <li><a href="fit_Method.html#a">Preliminary Experiment </a></li>
      <li><a href="fit_Method.html#b">Synthesis  of the "Barel" particles</a></li>
      <li><a href="fit_Method.html#c">Combining the "Doll" particles </a></li>
　　　<li><a href="fit_Method.html#d">Pop-up of the "Doll" particle</a></li>
　　　<li><a href="fit_Method.html#e">Material</a></li>
  </ul>
  </li>
  <li><a href="fit_Results and Discussion.html#">Result and Discassions</a>
    <ul>
      <li><a href="fit_Results and Discussion.html#b">Preliminary Experiment</a></li>
      <li><a href="fit_Results and Discussion.html#c">Synthesis of the “Barrel” particles and the “Doll” particles</a></li>
      <li><a href="fit_Results and Discussion.html#d">Pop-up of the doll particle</a></li>
　　　<li><a href="fit_Results and Discussion.html#e">Conclusion</a></li>
    </ul>
  </li>
  <li><a href="fit_Member.html#team">Team</a>
    <ul>
      <li><a href="fit_Member.html#men">Member</a></li>
      <li><a href="fit_Member.html#spo">Sponsor</a></li>
     
    </ul>
  </li>
</ul>


</div>


</div>
</body>
</html>


<a name="back"></a>
<div align="left" style="margin: 1px 60px;">
<center><font size="6" color="#000022" face="Arial"><b>Background&Motivation</b></font></center>


<font size="4" color="#000022" face="Arial">


<p>
<table align="left">
<td><div style="width: 330px;"><img alt="" src="/images/f/f6/Hy0.png" width="330" height="200" border="0" / ><p>Fig1.Pop-up pirate    </p><div></td><tr>
<td><div style="width: 330px;"><img alt="" src="/images/7/7b/Hy1-3.png" width="330" height="290" border="0" / ><p>Fig2. Application of the pop-up pirate for DDS: a comparative scheme.</p><div></td></table>


&nbsp;
<p>
&nbsp;&nbsp;  Pop-up pirate (Fig.1) is the toy which Japanese Takara Tommy started to sale in 1975. We first put a doll in a barrel.(Fig. 1a). The barrel of this toy has holes to stab a swords. Then we stab swords to the barrel one by one (Fig. 1b). When a player stabs the specific point on the barrel, the doll jumps out (Fig. 1c). The player who made the doll jump out is a loser. We think it interesting that the doll pops up only when the sword is stabbed in a specific position. The specific position changes at every games.<br><br>
&nbsp;We intended to tie this phenomenon to a scientific field. What will happen if we can realize a pop-up pirate on a nano-scale ? The nano-pop-up-pirate is regarded as a nano-robot which release a substances or trigger a mechanical movement by selectively recognizing a specific molecular information. The applications for molecular information amplification and drug delivery system (DDS) will be possible, which is scientifically and technically significant.
DDS (drug delivery system) is a general technique to transport a drug to an internal target part in the body or to control quantity of drug in the target part appropriately. In this system, we have three important points: no release of a drug before transporting the drug to the affected part, the targeting to an affected part, and release controlled by a specific stimulation. In comparison with a conventional drug transit technology, we can expect suppression of expression of the specific action, decrease in the burdens on patient by the reduction of the dose, reduced side effects, reduced medical expenses, and low cost. DDS materials made of the DNA-silica hybrids, which will be developed based on the present study, will be applicable for DDS systems with high stability and low cost (Fig. 2).




</p>


<hr>


<br>
<br>
<a name="goal"></a>
<center><font size="6" color="#000022" face="Arial"><b>Proiect goals</b></font></center><br><br>


<table align="center">
<td><div style="width: 330px;"><img alt="" src="/images/0/01/Hy2-2.png" width="330" height="280" border="0" / ><p>Fig. 3. The goals of our project <p></p><div></td><tr></table>
<p>
&nbsp;&nbsp;  A purpose of this study is to fabricate a pop-up pirate on the nano-scale. We synthesize colloidal particles and use them as the nano-doll and nano-barrel. We then combine the doll- and the barrel-particle by hybridization of the ssDNAs grafted on the particles. Finally we observe the release (pop-up) of the doll-particle in response to specific stimulation (adding "sword DNA" with fully complementary base sequence with the barrel DNA). We divide our goals into the 5 steps as shown in Fig. 3.<br>
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Latest revision as of 20:58, 25 October 2014

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<a name="header"></a> <img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">



  • <a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
  • <a href="fit_Introduction.html#pro">Projects</a>
    • <a href="fit_Introduction.html#back">Background & Motivation</a>
    • <a href="fit_Introduction.html#goal">Project Goals</a>
  • <a href="fit_Approach and Goals.html#des">Design</a>
    • <a href="fit_Approach and Goals.html#ear">Early Design</a>
    • <a href="fit_Approach and Goals.html#fin">Final Design</a>
  • <a href="fit_Method.html#met">Method</a>
    • <a href="fit_Method.html#a">Preliminary Experiment </a>
    • <a href="fit_Method.html#b">Synthesis of the "Barel" particles</a>
    • <a href="fit_Method.html#c">Combining the "Doll" particles </a>
      • 　　　
    • <a href="fit_Method.html#d">Pop-up of the "Doll" particle</a>
      • 　　　
    • <a href="fit_Method.html#e">Material</a>
  • <a href="fit_Results and Discussion.html#">Result and Discassions</a>
    • <a href="fit_Results and Discussion.html#b">Preliminary Experiment</a>
    • <a href="fit_Results and Discussion.html#c">Synthesis of the “Barrel” particles and the “Doll” particles</a>
    • <a href="fit_Results and Discussion.html#d">Pop-up of the doll particle</a>
      • 　　　
    • <a href="fit_Results and Discussion.html#e">Conclusion</a>
  • <a href="fit_Member.html#team">Team</a>
    • <a href="fit_Member.html#men">Member</a>
    • <a href="fit_Member.html#spo">Sponsor</a>


<a name="back"></a>

Background&Motivation

<img alt="" src="/images/f/f6/Hy0.png" width="330" height="200" border="0" / >

Fig1.Pop-up pirate

<img alt="" src="/images/7/7b/Hy1-3.png" width="330" height="290" border="0" / >

Fig2. Application of the pop-up pirate for DDS: a comparative scheme.

 

   Pop-up pirate (Fig.1) is the toy which Japanese Takara Tommy started to sale in 1975. We first put a doll in a barrel.(Fig. 1a). The barrel of this toy has holes to stab a swords. Then we stab swords to the barrel one by one (Fig. 1b). When a player stabs the specific point on the barrel, the doll jumps out (Fig. 1c). The player who made the doll jump out is a loser. We think it interesting that the doll pops up only when the sword is stabbed in a specific position. The specific position changes at every games.

 We intended to tie this phenomenon to a scientific field. What will happen if we can realize a pop-up pirate on a nano-scale ? The nano-pop-up-pirate is regarded as a nano-robot which release a substances or trigger a mechanical movement by selectively recognizing a specific molecular information. The applications for molecular information amplification and drug delivery system (DDS) will be possible, which is scientifically and technically significant. DDS (drug delivery system) is a general technique to transport a drug to an internal target part in the body or to control quantity of drug in the target part appropriately. In this system, we have three important points: no release of a drug before transporting the drug to the affected part, the targeting to an affected part, and release controlled by a specific stimulation. In comparison with a conventional drug transit technology, we can expect suppression of expression of the specific action, decrease in the burdens on patient by the reduction of the dose, reduced side effects, reduced medical expenses, and low cost. DDS materials made of the DNA-silica hybrids, which will be developed based on the present study, will be applicable for DDS systems with high stability and low cost (Fig. 2).



<a name="goal"></a>

Proiect goals


<img alt="" src="/images/0/01/Hy2-2.png" width="330" height="280" border="0" / >

Fig. 3. The goals of our project

   A purpose of this study is to fabricate a pop-up pirate on the nano-scale. We synthesize colloidal particles and use them as the nano-doll and nano-barrel. We then combine the doll- and the barrel-particle by hybridization of the ssDNAs grafted on the particles. Finally we observe the release (pop-up) of the doll-particle in response to specific stimulation (adding "sword DNA" with fully complementary base sequence with the barrel DNA). We divide our goals into the 5 steps as shown in Fig. 3.



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