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<body>
<div style="background: url(/images/4/46/Fitaoback.png);">
<img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">
<hr>
<br>
<ul id="dropmenu">
  <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
    <ul>
      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#abs">Abstract</a></li>
      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#vid">Video</a></li>
 
    </ul>
  </li>
  <li><a href="fit_Introduction.html#pro">Projects</a>
    <ul>
      <li><a href="fit_Introduction.html#back">background & Motivation</a></li>
      <li><a href="fit_Introduction.html#goal">Goals</a></li>
    </ul>
  </li>
  <li><a href="fit_Approach and Goals.html#des">Design</a>
    <ul>
      <li><a href="fit_Approach and Goals.html#ear">Early Design</a></li>
      <li><a href="fit_Approach and Goals.html#fin">Final Design</a></li>
     
    </ul>
  </li>
  <li><a href="fit_Method.html#met">Method</a>
    <ul>
      <li><a href="fit_Method.html#a">Barrel particle</a></li>
      <li><a href="fit_Method.html#b">DNA modified</a></li>
      <li><a href="fit_Method.html#c">樽と人形の結合</a></li>
　　　<li><a href="fit_Method.html#d">樽人形剣</a></li>
　　　<li><a href="fit_Method.html#e">Material</a></li>
  </ul>
  </li>
  <li><a href="fit_Results and Discussion.html#">Result and Discassions</a>
    <ul>
      <li><a href="fit_Results and Discussion.html#a">Barrel particle and Doll particle</a></li>
      <li><a href="fit_Results and Discussion.html#b">DNA modified</a></li>
      <li><a href="fit_Results and Discussion.html#c">樽と人形の結合</a></li>
      <li><a href="fit_Results and Discussion.html#d">樽人形剣</a></li>
　　　<li><a href="fit_Results and Discussion.html#e">Conclusion</a></li>
    </ul>
  </li>
  <li><a href="fit_Member.html#team">Team</a>
    <ul>
      <li><a href="fit_Member.html#men">Menber</a></li>
      <li><a href="fit_Member.html#spo">Sponsor</a></li>
     
    </ul>
  </li>
</ul>
<div align="center">
<font size="6" color="#000022" face="Arial"><b>Abstract</b></font>
<font size="4" color="#000022" face="Arial">
<p>
&nbsp;  aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa<br>
bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb<br>
<br><br><br><br><br>
</p>
</div>
</font>
<font size="3" color="#000022" face="Arial">
<center>
<table style="background-color:#ffffff;filter:alpha(opacity= 20);-moz-opacity: 0.5;opacity: 0.5;"
border="1" align="center" width="90%" height="1045" hspace="3" vspace="3" bordercolor="#666666">
<caption align="left">Material</caption>
<tr>
<th align="center" valign="middle" width="200" height="5">Structural formula</th>
<th align="center" valign="middle" width="200" height="5">Name</th>
<th align="center" valign="middle" width="200" height="5">Manufacturer</th>
<th align="center" valign="middle" width="100" height="5">Grade</th>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/2/2c/1.png.gif"/  width="160" height="100" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Sulfo-NHS</td>
<td align="center" valign="middle" width="200" height="130">DOUJINDO</td>
<td align="center" valign="middle" width="100" height="130">200mg
</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/3/3b/FIT%EF%BC%92.png.gif" width="180" height="90" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Tetraethyl orthosilicate<br>
(Tetraethoxysilane; TEOS)
</td>
<td align="center" valign="middle" width="200" height="130">Wako</td>
<td align="center" valign="middle" width="100" height="130">500mg</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/9/98/FIT%EF%BC%93.png.gif" width="180" height="90" border="" /></td>
<td align="center" valign="middle" width="200" height="50">3-aminopropyltriethoxysilane</td>
    <td align="center" valign="middle" width="200" height="50">Wako</td>
    <td align="center" valign="middle" width="100" height="50">25g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/1/1f/FIT%EF%BC%94.png.png" width="230" height="120" border=""/></td>
<td align="center" valign="middle" width="200" height="130">1 - (3-dimethylaminopropyl) carbodiimide hydrochloride</td>
<td align="center" valign="middle" width="200" height="130">TGI</td>
<td align="center" valign="middle" width="100" height="130">5g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/c/cf/FIT%E3%80%80%EF%BC%95.gif" width="150" height="100" border=""/></td>
<td align="center" valign="middle" width="200" height="130">N-N-dimethylformamide</td>
<td align="center" valign="middle" width="200" height="130">TGI</td>
<td align="center" valign="middle" width="100" height="130">200mg</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/0/0a/FIT_6.gif" width="145" height="90" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Succinic anhydride</td>
<td align="center" valign="middle" width="200" height="130">Wako</td>
<td align="center" valign="middle" width="100" height="130">25g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/7/73/FIT_7.gif" width="200" height="70" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Cetyltrimethylammonium bromide</td>
<td align="center" valign="middle" width="200" height="130">Wako</td>
<td align="center" valign="middle" width="100" height="130">25g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/c/c2/FIT_8.gif" width="180" height="90" border=""/></td>
<td align="center" valign="middle" width="200" height="130">MES
</td><td align="center" valign="middle" width="200" height="130">DOUJINDO
</td><td align="center" valign="middle" width="100" height="130">25g
</td>
</tr>
</table>
<br><br><br>
<table border="1" rules="rows" style="background-color:#ffffff;filter:alpha(opacity= 20);-moz-opacity: 0.5;opacity: 0.5;"
align="center" width="80%" height="200" hspace="3" vspace="3" bordercolor="#666666">
  <caption align="left">Table 3</caption>
  <tr>
  <th>Fluorescent molecule</th><th>Place</th><th>Excitation wavelength</th><th>Maximum fluorescence wavelength</th>
  </tr>
  <tr align="center" valign="middle">
  <td>TAMRA(red)</td><td>Sword</td><td>550nm</td><td>580nm</td>
  </tr>
  <tr align="center" valign="middle">
  <td>FITC(green)</td><td>Barrel</td><td>498nm</td><td>522nm</td>
  </tr>
</table>
<br><br><br>
<table style="background-color:#ffffff;filter:alpha(opacity= 20);-moz-opacity: 0.5;opacity: 0.5;"  border="1" align="center" width="90%" height="440" hspace="3" vspace="3" bordercolor="#666666">
<caption align="left">Apparatus</caption>
<tr>
<th align="center" valign="middle" width="100" height="50">Device name</th>
<th align="center" valign="middle" width="100" height="50">Manufacturer
Model number
</th>
<th align="center" valign="middle" width="200" height="50">Measurement condition </th>
<th align="center" valign="middle" width="200" height="50">Photograph</th>
        <th align="center" valign="middle" width="200" height="50">Explanations of equipment</th>
</tr>
<tr>
<td align="center" valign="middle" width="100" height="130">Spectrophotometer</td>
<td align="center" valign="middle" width="100" height="130">HITACHI F-2500</td>
<td align="center" valign="middle" width="200" height="130">WL Range:
450nm~650nm
Scanning Speed:
60nm/min
  </td>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/6/6d/FIT_9.png" width="150" height="130" border=""/>  </td>
　　　　　<td align="center" valign="middle" width="200" height="130">This device can see the concentration of the solution, the material characteristics, the structure of the molecule.  </td>
</tr>
<tr>
<td align="center" valign="middle" width="100" height="130">SEM</td>
<td align="center" valign="middle" width="100" height="130">KEYENC VE-7800</td>
<td align="center" valign="middle" width="200" height="130">WL Range:
450nm~650nm
Scanning Speed:
60nm/min
  </td>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/9/9f/FIT_10.png" width="150" height="130" border="">  </td>
　　　　　<td align="center" valign="middle" width="200" height="130">It is possible to significantly change the magnification easily. In addition, photography is possible.  </td>
</tr>
<tr>
<td align="center" valign="middle" width="100" height="130">Confocal laser scanning microscope
(CLSM)
</td>
<td align="center" valign="middle" width="100" height="130">Nikon ECLIPSE-Ti</td>
<td align="center" valign="middle" width="200" height="130">Ar-Laser (488nm)
Garvano mirror scanner
  </td>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/8/8e/FIT_11.png" width="150" height="130" border=""></td>
　　　　　<td align="center" valign="middle" width="200" height="130">It is possible to obtain a high resolution image in focus in the field of view for the entire sample surface with irregularities.
Three-dimensional shape measurement non-destructive, non-contact is possible
  </td>
</tr>
</table>


</center>


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<ul id="menu" style="list-style:none;">
<li><a href="#">TOP</a></li>
<li><a href="fit_Introduction.html">Intro</a>
<ul class="sub"style="list-style:none;">
<li><a href="fit_Introduction.html#How">How to play kurohige</a></li>
<li><a href="fit_Introduction.html#Why">Why kurhige</a></li>
<li><a href="fit_Introduction.html#Pro">problem</a></li>
</ul>
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<li><a href="fit_Approach and Goals.html">Approach</a>
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<li><a href="fit_Approach and Goals.html#DNA">DNA</a></li>
<li><a href="fit_Approach and Goals.html#Silica">Silica</a></li>
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<li><a href="fit_Method.html#DNA">DNA</a></li>
<li><a href="fit_Method.html#Silica">Silica</a></li>
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<li><a href="fit_Method.html#DNA-Silica">DNA-Silica</a></li>
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<li><a href="fit_Results and Discussion.html">result</a>
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<li><a href="fit_Results and Discussion.html#DNA">DNA</a></li>
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Latest revision as of 17:43, 25 October 2014

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<img src="/images/4/46/Fitaologo.PNG" alt" alt="" width="422" height="98" hspace="0" align="left">



  • <a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
  • <a href="fit_Introduction.html#pro">Projects</a>
    • <a href="fit_Introduction.html#back">background & Motivation</a>
    • <a href="fit_Introduction.html#goal">Goals</a>
  • <a href="fit_Approach and Goals.html#des">Design</a>
    • <a href="fit_Approach and Goals.html#ear">Early Design</a>
    • <a href="fit_Approach and Goals.html#fin">Final Design</a>
  • <a href="fit_Method.html#met">Method</a>
    • <a href="fit_Method.html#a">Barrel particle</a>
    • <a href="fit_Method.html#b">DNA modified</a>
    • <a href="fit_Method.html#c">樽と人形の結合</a>
      • 　　　
    • <a href="fit_Method.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Method.html#e">Material</a>
  • <a href="fit_Results and Discussion.html#">Result and Discassions</a>
    • <a href="fit_Results and Discussion.html#a">Barrel particle and Doll particle</a>
    • <a href="fit_Results and Discussion.html#b">DNA modified</a>
    • <a href="fit_Results and Discussion.html#c">樽と人形の結合</a>
    • <a href="fit_Results and Discussion.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Results and Discussion.html#e">Conclusion</a>
  • <a href="fit_Member.html#team">Team</a>
    • <a href="fit_Member.html#men">Menber</a>
    • <a href="fit_Member.html#spo">Sponsor</a>



Abstract

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Material
Structural formula Name Manufacturer Grade
<img alt="" src="/images/2/2c/1.png.gif"/ width="160" height="100" border=""/> Sulfo-NHS DOUJINDO 200mg
<img alt="" src="/images/3/3b/FIT%EF%BC%92.png.gif" width="180" height="90" border=""/> Tetraethyl orthosilicate

(Tetraethoxysilane; TEOS)

Wako 500mg
<img alt="" src="/images/9/98/FIT%EF%BC%93.png.gif" width="180" height="90" border="" /> 3-aminopropyltriethoxysilane Wako 25g
<img alt="" src="/images/1/1f/FIT%EF%BC%94.png.png" width="230" height="120" border=""/> 1 - (3-dimethylaminopropyl) carbodiimide hydrochloride TGI 5g
<img alt="" src="/images/c/cf/FIT%E3%80%80%EF%BC%95.gif" width="150" height="100" border=""/> N-N-dimethylformamide TGI 200mg
<img alt="" src="/images/0/0a/FIT_6.gif" width="145" height="90" border=""/> Succinic anhydride Wako 25g
<img alt="" src="/images/7/73/FIT_7.gif" width="200" height="70" border=""/> Cetyltrimethylammonium bromide Wako 25g
<img alt="" src="/images/c/c2/FIT_8.gif" width="180" height="90" border=""/> MES DOUJINDO 25g




Table 3
Fluorescent moleculePlaceExcitation wavelengthMaximum fluorescence wavelength
TAMRA(red)Sword550nm580nm
FITC(green)Barrel498nm522nm




　　　　　 　　　　　 　　　　　
Apparatus
Device name Manufacturer

Model number

Measurement condition Photograph Explanations of equipment
Spectrophotometer HITACHI F-2500 WL Range:

450nm~650nm Scanning Speed: 60nm/min

<img alt="" src="/images/6/6d/FIT_9.png" width="150" height="130" border=""/> This device can see the concentration of the solution, the material characteristics, the structure of the molecule.
SEM KEYENC VE-7800 WL Range:

450nm~650nm Scanning Speed: 60nm/min

<img alt="" src="/images/9/9f/FIT_10.png" width="150" height="130" border=""> It is possible to significantly change the magnification easily. In addition, photography is possible.
Confocal laser scanning microscope

(CLSM)

Nikon ECLIPSE-Ti Ar-Laser (488nm)

Garvano mirror scanner

<img alt="" src="/images/8/8e/FIT_11.png" width="150" height="130" border="">It is possible to obtain a high resolution image in focus in the field of view for the entire sample surface with irregularities.

Three-dimensional shape measurement non-destructive, non-contact is possible






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