Biomolecular Breadboards:More Info: Difference between revisions
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==== References ==== | ==== References ==== | ||
* D. Siegal-Gaskins, V. Noireaux and Richard M. Murray, [http://www.cds.caltech.edu/~murray/papers/snm13-acc.html Biomolecular resource utilization in elementary cell-free gene circuits]. | * Z. Z. Sun, C. A. Hayes, J. Shin, F. Caschera, R. M. Murray, V. Noireaux, Protocols for Implementing an Escherichia Coli Based TX-TL Cell-Free Expression System for Synthetic Biology. ''Journal of Visualized Experiments'', 2013 (to appear). | ||
* D. Siegal-Gaskins, V. Noireaux and Richard M. Murray, [http://www.cds.caltech.edu/~murray/papers/snm13-acc.html Biomolecular resource utilization in elementary cell-free gene circuits]. 2013 American Control Conference. | |||
* J. Shin and V. Noireaux, [http://pubs.acs.org/doi/abs/10.1021/sb200016s An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells]. ''ACS Synthetic Biology'', 1(1):29–41, 2012. | * J. Shin and V. Noireaux, [http://pubs.acs.org/doi/abs/10.1021/sb200016s An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells]. ''ACS Synthetic Biology'', 1(1):29–41, 2012. | ||
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* [http://www.cds.caltech.edu/~murray/talks/breadboard_regional-25Oct12.pdf DARPA Living Foundries west coast presentation], 25 Oct 2012 | * [http://www.cds.caltech.edu/~murray/talks/breadboard_regional-25Oct12.pdf DARPA Living Foundries west coast presentation], 25 Oct 2012 | ||
* [http://www.cds.caltech.edu/~murray/talks/breadboard_kickoff-12Jul12.pdf DARPA Living Foundries kickoff presentation], 12 Jul 2012 | * [http://www.cds.caltech.edu/~murray/talks/breadboard_kickoff-12Jul12.pdf DARPA Living Foundries kickoff presentation], 12 Jul 2012 | ||
==== Videos ==== | |||
* [https://www.youtube.com/watch?v=ER4qAIQy-wU TXTL v4.1 1/2 Template Setup], YouTube, Sep 2012 | |||
* [https://www.youtube.com/watch?v=35eOX2GqwAM TXTL v4.1 2/2 - running reactions], YouTube, Sep 2012 | |||
Revision as of 02:10, 11 July 2013
| Home | Protocols | DNA parts | Preliminary Data | Models | More Info |
The cell-free circuit breadboard, artificial cell breadboard and DNA origami breadboards are being developed at Caltech and U. Minnesota under the support of the DARPA Living Foundries program. For more information, contact:
Richard Murray
California Institute of Technology
1200 E California Blvd
Pasadena, CA 91125
http://www.cds.caltech.edu/~murray/wiki/Main_Page
References
- Z. Z. Sun, C. A. Hayes, J. Shin, F. Caschera, R. M. Murray, V. Noireaux, Protocols for Implementing an Escherichia Coli Based TX-TL Cell-Free Expression System for Synthetic Biology. Journal of Visualized Experiments, 2013 (to appear).
- D. Siegal-Gaskins, V. Noireaux and Richard M. Murray, Biomolecular resource utilization in elementary cell-free gene circuits. 2013 American Control Conference.
- J. Shin and V. Noireaux, An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells. ACS Synthetic Biology, 1(1):29–41, 2012.
- E. Karzbrun, J. Shin, R. Bar-Ziv and V. Noireaux, Coarse-grained dynamics of protein synthesis in a cell-free system. Phys. Rev. Lett., 106(4);048104, 2011.
- J. Shin and V. Noireaux, Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system. J Biol Eng, 4;9, 2010.
- J. Shin and V. Noireaux, Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. J Biol Eng, 4;8, 2010.
Presentations
- SB 6.0 presentation, 09 Jul 2013
- UC Berkeley Bioengineering Seminar, 08 May 2013
- DARPA Living Foundries midyear lightning talk, 20 Feb 2013
- DARPA Living Foundries east coast presentation, 6 Nov 2012
- DARPA Living Foundries west coast presentation, 25 Oct 2012
- DARPA Living Foundries kickoff presentation, 12 Jul 2012
Videos
- TXTL v4.1 1/2 Template Setup, YouTube, Sep 2012
- TXTL v4.1 2/2 - running reactions, YouTube, Sep 2012
