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Cell-free circuit breadboard
The following protocols are available for the cell-free circuit breadboard:
- Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
The cell-free circuit breadboard costs approximately 1.05 cents per uL, or 12.6 cents per typical 12 uL reaction. The cost distribution is shown in the table below.
|Component||Price per μL of reaction||Price per typical 12 μL reaction|
|E. coli crude extract||0.45 cents||5.4 cents|
|Amino acids||0.21 cents||2.5 cents|
|3-PGA||0.39 cents||4.7 cents|
|Total price||1.05 cents||12.6 cents|
For a more detailed cost estimate, please see Breadboard Cost Estimate.