Biomolecular Breadboards:Protocols
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Cell-free circuit breadboard
The following protocols are available for the cell-free circuit breadboard:
- Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
Cost estimate
The cell-free circuit breadboard costs approximately 1.05 cents per uL, or 12.6 cents per typical 12 uL reaction. The cost distribution is shown in the table below.
| Component | Price per μL of reaction | Price per typical 12 μL reaction |
|---|---|---|
| E. coli crude extract | 0.45 cents | 5.4 cents |
| Amino acids | 0.21 cents | 2.5 cents |
| 3-PGA | 0.39 cents | 4.7 cents |
| Total price | 1.05 cents | 12.6 cents |
For a more detailed cost estimate, please see Breadboard Cost Estimate.
