Biomolecular Breadboards:Protocols

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Cell-free circuit breadboard

The following protocols are available for the cell-free circuit breadboard:

• Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
  • E. Coli Crude Extract Preparation, 3X
  • Amino Acid Preparation
  • 3-PGA Buffer Preparation, 14X

Cost estimate

The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.

Component	Material price per μL of reaction	Labor price per μL of reaction	Total price per μL of reaction	Total price per 12 μL reaction
E. coli crude extract	0.45 cents	0.62 cents	1.07 cents	12.84 cents
Amino acids	0.21 cents	0.034 cents	0.244 cents	2.93 cents
3-PGA	0.39 cents	0.94 cents	1.33 cents	15.96 cents
Total price	1.05 cents	1.6 cents	2.64 cents	31.7 cents

For a more detailed cost estimate, please see Breadboard Cost Estimate.