Biomolecular Breadboards:Protocols
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Cell-free circuit breadboard
The following protocols are available for the cell-free circuit breadboard:
- Reaction Preparation - Cell-free expression of circuits from linear or plasmid DNA
- TXTL template, v2.3 - TXTL spreadsheet for designing experiments
Cost estimate
The cell-free circuit breadboard costs approximately 2.64 cents per uL, or 31.7 cents per 12 uL reaction. The cost distribution is shown in the table below.
Component | Material price per
μL of reaction | Labor price per
μL of reaction | Total price per
μL of reaction | Total price per
12 μL reaction |
---|---|---|---|---|
E. coli crude extract | 0.45 cents | 0.62 cents | 1.07 cents | 12.84 cents |
Amino acids | 0.21 cents | 0.03 cents | 0.24 cents | 2.93 cents |
3-PGA | 0.39 cents | 0.94 cents | 1.33 cents | 15.96 cents |
Total price | 1.05 cents | 1.6 cents | 2.64 cents | 31.7 cents |
For a more detailed cost estimate, please see Breadboard Cost Estimate.
Design Cycle Time Analysis
For a more detail description, please see Breadboard Cycle Time Measurements.