Biomolecular Breadboards:Protocols:3-PGA Buffer Prep: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Clare Hayes (talk | contribs) mNo edit summary |
Clare Hayes (talk | contribs) mNo edit summary |
||
| Line 30: | Line 30: | ||
|- | |- | ||
| 3-PGA || 1.4M || 30mM || 128.6µl || 514.4µl || 771.6µl | | 3-PGA || 1.4M || 30mM || 128.6µl || 514.4µl || 771.6µl | ||
|} | |||
{| border="1" cellpadding="2" style="width: 600px" | |||
| style="width: 350px;" | Total Volume || style="width: 75px;" | 427µl || style="width: 75px;" | 1708µl || style="width: 75px;" | 2562µl | |||
|} | |} | ||
Revision as of 18:32, 5 June 2012
| Home | Protocols | DNA parts | Preliminary Data | Models | More Info |
Preparation of 3-PGA Buffer for the Cell-Free Expression System, 14X
| Component | Stock | Final Concentration in cell-free reaction | 1-fold | 4-fold | 6-fold |
|---|---|---|---|---|---|
| HEPES pH8 | 2M | 50mM | 150µl | 600µl | 900µl |
| Water | 6µl | 24µl | 36µl | ||
| Homemade Nucleotides | 156mM: ATP & GTP 94mM: CTP & UTP | 1.5mM: ATP & GTP 0.9mM: CTP & UTP | 57.8µl | 231.2µl | 346.8µl |
| tRNA E.coli | 50mg/ml | 0.2mg/ml | 24µl | 96µl | 144µl |
| CoA | 65mM | 0.26mM | 24µl | 96µl | 144µl |
| NAD | 175mM | 0.33mM | 11.5µl | 46µl | 69µl |
| cAMP | 650mM | 0.75mM | 7.1µl | 28.4µl | 42.6µl |
| Folinic Acid | 33.9mM | 0.068mM | 12µl | 48µl | 72µl |
| Spermidine | 1M | 1mM | 6µl | 24µl | 36µl |
| 3-PGA | 1.4M | 30mM | 128.6µl | 514.4µl | 771.6µl |
| Total Volume | 427µl | 1708µl | 2562µl |
