Biomolecular Breadboards:Protocols:cost estimate: Difference between revisions
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{{Template:Biomolecular Breadboards}} | |||
== Cell-Free Circuit Breadboard Cost Estimate == | == Cell-Free Circuit Breadboard Cost Estimate == | ||
Revision as of 21:26, 11 August 2012
| Home | Protocols | DNA parts | Preliminary Data | Models | More Info |
Cell-Free Circuit Breadboard Cost Estimate
Reaction Ingredients
| Component | % Reaction Volume | Price per uL of reaction | Price per 12 uL reaction | Source | Catalog Number
|
|---|---|---|---|---|---|
| Extract | 33.3% | $0.00448 | $0.05376 | Homemade | |
| Amino acids | 25% | $0.0021 | $0.0252 | Homemade | |
| 3-PGA buffer | 7.14% | $0.00391 | $0.04697 | Homemade | |
| Mg-glutamate | 1% | negligible | Sigma-Aldrich | 49605 | |
| K-glutamate | 1.3% | negligible | Sigma-Aldrich | G1149 | |
| DTT | 1% | negligible | Sigma-Aldrich | D0632 | |
| PEG8000 | 5% | negligible | Promega | V3011 | |
| DNA, water, inducer | 26.26% | User-supplied | |||
| Total Cost | $0.0105 | $0.126 |
E. coli crude extract
"Amount per 3X prep" refers to the amount of crude extract made following the protocol E. Coli Crude Extract Preparation, 3X. On average, one prep yields 10.5 mL of extract, which is enough for about 31500 uLs of cell-free circuit reaction.
| Ingredient | Bulk Quantity | Bulk Price | Amount per 3X prep | Price per 3X prep | Source | Catalog Number |
|---|---|---|---|---|---|---|
| 2xYT | 1 kg | $87.3 | 139.93 g | $12.216 | MP biomedicals | 3012-032 |
| Bacto-Agar | 1 lb | $171.11 | 0.44 g | $0.17 | BD (from Fisher) | 214010 |
| Chloramphenicol powder | 5 g | $30.50 | 3.876 mg | $0.02 | Sigma-Aldrich | C1919 |
| DTT | 5 g | $97.40 | 0.927 g | $18.06 | Sigma-Aldrich | D0632 |
| Ethanol (100%) | 500 mL | $54.68 | 114 uL | $0.01 | Fisher | BP2818 |
| K-glutamate | 500 g | $161.50 | 48.8 g | $15.76 | Sigma-Aldrich | G1149 |
| Mg-glutamate | 250 g | $47.10 | 21.76 g | $4.10 | Sigma-Aldrich | 49605 |
| Potassium phosphate monobasic solution | 1 L | $43.80 | 90.15 mL | $3.95 | Sigma-Aldrich | P8709 |
| Potassium phosphate dibasic solution | 1 L | $38.00 | 163.92 mL | $6.23 | Sigma-Aldrich | P8584 |
| Tris | 1 kg | $133.58 | 13.325 g | $1.78 | Fisher | BP1521 |
| Total Price | $62.30 |
| Disposable Material | Bulk Quantity | Bulk Price | Amount per 3X prep | Price per 3X prep | Source | Catalog Number |
|---|---|---|---|---|---|---|
| 2xYT | 1 kg | $87.3 | 139.93 g | $12.216 | MP Biomedicals | 3012-032 |
| Beads (0.1 mm diameter) | 1 lb | $34.00 | 80 g | $6.00 | BioSpec | 11079101 |
| Bead beating tubes | 500 count | $141.00 | 42 count | $11.84 | BioSpec | 522S |
| Bead filters | 100 count | $112.00 | 6 count | $6.72 | Bio-Rad | 732-6204 |
| Bio-rad Bradford protein assay kit (BSA) | 1 kit | $123.00 | 1 assay | $0.62 | Bio-Rad | 500-0201 |
| Cuvettes | 500 count | $170.30 | 7 count | $2.38 | Fisher | 14-955-127 |
| Dialysis cassettes | 10 count | $85.40 | 6 count | $51.24 | Thermo Scientific (from Fisher) | 66380 |
| Total Price | $78.80 |
| Total Price of E. coli crude extract, 3x Prep (Ingredients + Disposable Materials) | $141.10 |
| Price per uL of reaction | $0.00448 |
| Price per 12 uL reaction | $0.05376 |
Amino acids
One kit yields about 41.6 mL of amino acids, which is enough for approximately 166,400 uLs of cell-free circuit reaction. See Amino Acid Preparation for instructions on how to prepare the kit.
| Ingredient | Quantity | Price | Price per uL of reaction | Price per 12 uL reaction | Source | Catalog Number |
|---|---|---|---|---|---|---|
| RTS Amino Acid Sampler | 1 kit | $349.00 | $0.0021 | $0.0252 | 5 Prime | 2401530 |
3-PGA buffer
"Amount per 1-fold prep" refers to the amount of amino acids made following the instructions for 1-fold preparation of 3-PGA buffer in the protocol 3-PGA Buffer Preparation, 14X. 1-fold preparation yields 427 uL of 3-PGA buffer, which is enough for 5977 uLs of cell-free circuit reaction.
| Ingredient | Bulk Quantity | Bulk Price | Amount per
1-fold prep |
Price per
1-fold prep |
Source | Catalog Number |
|---|---|---|---|---|---|---|
| HEPES KOH ph8 | 100 g | $156.50 | 71.493 mg | $0.11 | Sigma Aldrich | H6147 |
| ATP | 1 g | $51.50 | 5.6 mg | $0.29 | Sigma Aldrich | A8937 |
| GTP | 1 g | $767.00 | 5.155 mg | $3.95 | USB (from Affymetrix) | 16800 |
| UTP | 1 g | $177.00 | 3.19 mg | $0.56 | USB (from Affymetrix) | 23160 |
| CTP | 1 g | $298.00 | 3.0727 mg | $0.92 | USB (from Affymetrix) | 14121 |
| tRNA (E. coli) | 100 mg | $148.00 | 1.2 mg | $1.78 | Roche Applied Science | MRE600 |
| CoA | 25 mg | $81.80 | 1.2 mg | $3.93 | Sigma Aldrich | C4282 |
| NAD | 250 mg | $37.60 | 1.43 mg | $0.22 | Sigma Aldrich | N6522 |
| cAMP | 1 g | $185.50 | 1.76 mg | $0.33 | Sigma Aldrich | A9501 |
| Folinic Acid | 100 mg | $64.60 | 0.208 mg | $0.13 | Sigma Aldrich | F7878 |
| Spermidine | 1 g | $50.80 | 0.000871 g | $0.04 | Sigma Aldrich | 85558 |
| 3-PGA | 1 g | $230.50 | 0.0476 g | $10.97 | Sigma Aldrich | P8877 |
| Tris | 1 kg | $133.58 | 1.302 g | $0.17 | Fisher | BP1521 |
| Total Price of 3-PGA buffer, 1-fold prep | $23.40 |
| Price per uL of reaction | $0.00391 |
| Price per 12 uL reaction | $0.04697 |
Vendor Links
| 5 Prime | |
| Affymetrix | http://www.affymetrix.com |
| Beckman Coulter | https://www.beckmancoulter.com |
| Bio-Rad | http://www.bio-rad.com/ |
| BioSpec | http://www.biospec.com/ |
| EMD Millipore | http://www.emdmillipore.com/ |
| Fisher | http://www.fishersci.com/ |
| MP Biomedicals | http://www.mpbio.com/ |
| Promega | http://www.promega.com/ |
| Roche Applied Science | http://www.roche-applied-science.com/ |
| Sigma Aldrich | http://www.sigmaaldrich.com/ |
