Biomolecular Breadboards:Protocols:cost estimate: Difference between revisions

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===Amino Acids===
===Amino Acids===
One kit yields about 41.6 mL of amino acids, which is enough for approximately 166,400 cell-free circuit reactions.  See [[Biomolecular Breadboards:Protocols:Amino Acids Prep|Amino Acid Preparation]] for instructions on kit preparation.
One kit yields about 41.6 mL of amino acids, which is enough for approximately 166,400 cell-free circuit reactions.  See [[Biomolecular Breadboards:Protocols:Amino Acids Prep|Amino Acid Preparation]] for instructions on how to prepare the kit.


{| border="1" cellpadding="5" style="width: 1050px"
{| border="1" cellpadding="5" style="width: 1050px"
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|-
| style="width: 250px;" | RTS Amino Acid Sampler || style="width: 100px;" | 1 kit || style="width: 100px;" | $349.00 || style="width: 100px;" | '''$0.0021''' || style="width: 100px;" | '''$0.0252''' || style="width: 200px;" | 5 Prime || style="width: 200 px;" | 2401530
| style="width: 250px;" | RTS Amino Acid Sampler || style="width: 100px;" | 1 kit || style="width: 100px;" | $349.00 || style="width: 100px;" | '''$0.0021''' || style="width: 100px;" | '''$0.0252''' || style="width: 200px;" | 5 Prime || style="width: 200 px;" | 2401530
|}
===3-PGA===
{| border="1" cellpadding="5" style="width: 1050px"
! scope="col" | Ingredient
! scope="col" | Bulk Quantity
! scope="col" | Bulk Price
! scope="col" | Amount per         
1-fold prep
! scope="col" | Price per
1-fold prep
! scope="col" | Source
! scope="col" | Catalog Number
|-
| style="width: 250px;" | HEPES KOH ph8 || style="width: 100px;" | 100 g || style="width: 100px;" | $156.50 || style="width: 100px;" | 71.493 mg || style="width: 100px;" | $0.11 || style="width: 200px;" | Sigma Aldrich || style="width: 200 px;" | H6147
|-
| ATP || 1 g || $51.50 || 5.6 mg || $0.29 || Sigma Aldrich || A8937
|-
| GTP || 1 g  || $767.00 || 5.155 mg || $3.95 || USB (from Affymetrix) || 16800
|-
| UTP || 1 g  || $177.00 || 3.19 mg || $0.56 || USB (from Affymetrix) || 23160
|-
| CTP || 1 g || $298.00 || 3.0727 mg || $0.92 || USB (from Affymetrix) || 14121
|-
| tRNA (''E. coli'') || 100 mg || $148.00 || 1.2 mg || $1.78 || Roche Applied Science || MRE600
|-
| CoA || 25 mg || $81.80 || 1.2 mg || $3.93 || Sigma Aldrich || C4282
|-
| NAD || 250 mg || $37.60 || 1.43 mg || $0.22 || Sigma Aldrich || N6522
|-
| cAMP || 1 g || $185.50 || 1.76 mg || $0.33 || Sigma Aldrich || A9501
|-
| Folinic Acid || 100 mg || $64.60 || 0.208 mg || $0.13 || Sigma Aldrich || F7878
|-
| Spermidine || 1 g || $50.80 || 0.000871 g || $0.04 || Sigma Aldrich || 85558
|-
| 3-PGA || 1 g || $230.50 || 0.0476 g || $10.97  || Sigma Aldrich || P8877
|-
| Tris || 1 kg || $133.58  || 1.302 g || $0.17 || Fisher || BP1521
|}
|}

Revision as of 19:37, 11 August 2012

Cell-Free Circuit Breadboard Cost Estimate

Reaction

E. coli crude extract

"Amount per 3X prep" refers to the amount of crude extract you would make following the protocol E. Coli Crude Extract Preparation, 3X. On average, one prep yields 10.5 mL of extract, which is enough for about 31500 cell-free circuit reactions.

Ingredient Bulk Quantity Bulk Price Amount per 3X prep Price per 3X prep Source Catalog Number
2xYT 1 kg $87.3 139.93 g $12.216 MP biomedicals 3012-032
Bacto-Agar 1 lb $171.11 0.44 g $0.17 BD (from Fisher) 214010
Chloramphenicol powder 5 g $30.50 3.876 mg $0.02 Sigma-Aldrich C1919
DTT 5 g $97.40 0.927 g $18.06 Sigma-Aldrich D0632
Ethanol (100%) 500 mL $54.68 114 uL $0.01 Fisher BP2818
K-glutamate 500 g $161.50 48.8 g $15.76 Sigma-Aldrich G1149
Mg-glutamate 250 g $47.10 21.76 g $4.10 Sigma-Aldrich 49605
Potassium phosphate monobasic solution 1 L $43.80 90.15 mL $3.95 Sigma-Aldrich P8709
Potassium phosphate dibasic solution 1 L $38.00 163.92 mL $6.23 Sigma-Aldrich P8584
Tris 1 kg $133.58 13.325 g $1.78 Fisher BP1521
Total Price $62.30



Disposable Material Bulk Quantity Bulk Price Amount per 3X prep Price per 3X prep Source Catalog Number
2xYT 1 kg $87.3 139.93 g $12.216 MP biomedicals 3012-032
Beads (0.1 mm diameter) 1 lb $34.00 80 g $6.00 BioSpec 11079101
Bead beating tubes 500 count $141.00 42 count $11.84 BioSpec 522S
Bead filters 100 count $112.00 6 count $6.72 Bio-Rad 732-6204
Bio-rad Bradford protein assay kit (BSA) 1 kit $123.00 1 assay $0.62 Bio-Rad 500-0201
Cuvettes 500 count $170.30 7 count $2.38 Fisher 14-955-127
Dialysis cassettes 10 count $85.40 6 count $51.24 Thermo Scientific (from Fisher) 66380
Total Price $78.80



Total Price of E. coli crude extract, 3x Prep (Ingredients + Disposable Materials) $141.10
Price per uL of reaction $0.00448
Price per 12 uL reaction $0.05376



Amino Acids

One kit yields about 41.6 mL of amino acids, which is enough for approximately 166,400 cell-free circuit reactions. See Amino Acid Preparation for instructions on how to prepare the kit.

Ingredient Quantity Price Price per uL of reaction Price per 12 uL reaction Source Catalog Number
RTS Amino Acid Sampler 1 kit $349.00 $0.0021 $0.0252 5 Prime 2401530


3-PGA

Ingredient Bulk Quantity Bulk Price Amount per

1-fold prep

Price per

1-fold prep

Source Catalog Number
HEPES KOH ph8 100 g $156.50 71.493 mg $0.11 Sigma Aldrich H6147
ATP 1 g $51.50 5.6 mg $0.29 Sigma Aldrich A8937
GTP 1 g $767.00 5.155 mg $3.95 USB (from Affymetrix) 16800
UTP 1 g $177.00 3.19 mg $0.56 USB (from Affymetrix) 23160
CTP 1 g $298.00 3.0727 mg $0.92 USB (from Affymetrix) 14121
tRNA (E. coli) 100 mg $148.00 1.2 mg $1.78 Roche Applied Science MRE600
CoA 25 mg $81.80 1.2 mg $3.93 Sigma Aldrich C4282
NAD 250 mg $37.60 1.43 mg $0.22 Sigma Aldrich N6522
cAMP 1 g $185.50 1.76 mg $0.33 Sigma Aldrich A9501
Folinic Acid 100 mg $64.60 0.208 mg $0.13 Sigma Aldrich F7878
Spermidine 1 g $50.80 0.000871 g $0.04 Sigma Aldrich 85558
3-PGA 1 g $230.50 0.0476 g $10.97 Sigma Aldrich P8877
Tris 1 kg $133.58 1.302 g $0.17 Fisher BP1521
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